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Information Journal Paper

Title

Molecular Identification and Genotyping of Acanthamoeba Isolated from Environmental Sources in Kashan, Central Iran

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Abstract

 Background: Acanthamoeba is one of the mostcommonopportunistic free-living amoebae, with ubiquitous presence in various environmental sources. Pathogenic strains of Acanthamoeba are the causative agents of amoebic keratitisandgranulomatous amoebic encephalitis. Objectives: The aim of the present study was to identify Acanthamoeba Genotypes in Soil, Hospital Dust, and stagnant water samples from Kashan, Central Iran. Methods: In this cross sectional study, a total of 122 samples from Soil (n, 32), Hospital Dust (n, 40), and stagnant water (n, 50) were collected and examined for the presence of free-living amoebae and Acanthamoeba species. All the samples were cultured onto non-nutrient agar plates for detection of free-living amoebae. Acanthamoeba species was identified by polymerase chain reaction (PCR) assay, using specific primers. A total of 29 Acanthamoeba isolates were sequenced, and different Genotypes were detected via sequence analysis. Results: The results showed that 82. 8% (101/122) of samples were positive for free-living amoebae. The PCR assay revealed that 62. 5%, 52. 5%, and 50% of Soil, Hospital Dust, and stagnant water samples were positive for Acanthamoeba species, respectively. Moreover, T4, T5, T2, T7, and T11 Genotypes were identified. The most common Genotype was T4 (76%), isolated from stagnant water. Conclusions: Acanthamoeba is a prevalent species in the Soil, Hospital Dust, and stagnant water of Kashan. As this protozoon can cause severe infections, health education and improvement of sanitation services are recommended for prevention of infection.

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    APA: Copy

    Golestani, Mohammad Hossein, RASTI, SIMA, HOOSHYAR, HOSSEIN, DELAVARI, MAHDI, MOUSAVI, SEYED GHOLAM ABBAS, IRANSHAHI, LEILA, & AGHAJANI, ALI. (2018). Molecular Identification and Genotyping of Acanthamoeba Isolated from Environmental Sources in Kashan, Central Iran. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 11(4), 0-0. SID. https://sid.ir/paper/317932/en

    Vancouver: Copy

    Golestani Mohammad Hossein, RASTI SIMA, HOOSHYAR HOSSEIN, DELAVARI MAHDI, MOUSAVI SEYED GHOLAM ABBAS, IRANSHAHI LEILA, AGHAJANI ALI. Molecular Identification and Genotyping of Acanthamoeba Isolated from Environmental Sources in Kashan, Central Iran. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2018;11(4):0-0. Available from: https://sid.ir/paper/317932/en

    IEEE: Copy

    Mohammad Hossein Golestani, SIMA RASTI, HOSSEIN HOOSHYAR, MAHDI DELAVARI, SEYED GHOLAM ABBAS MOUSAVI, LEILA IRANSHAHI, and ALI AGHAJANI, “Molecular Identification and Genotyping of Acanthamoeba Isolated from Environmental Sources in Kashan, Central Iran,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 11, no. 4, pp. 0–0, 2018, [Online]. Available: https://sid.ir/paper/317932/en

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