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Information Journal Paper

Title

Fibroblast-myofibroblast crosstalk after exposure to mesenchymal stem cells secretome

Pages

  73-79

Abstract

 Aim: The aim of the present study was to investigate the effect of human bone marrow-derived mesenchymal stem cells conditioned medium on Fibroblast to myoFibroblast differentiation. Background: Mesenchymal stem cells have a long-term clinical application and widely have used in autoimmune disease and regenerative medicine. However, some MSCs derived cytokines such as TGF-β could have a dual role in suppression or progression of disease. Fibroblast activation and extracellular matrix production are two key features of wound healing which mostly are controlled with multifunctional cytokine TGF-β 1. Methods: Bone marrow MSCs were isolated, cultured and used for conditioned medium preparation. The flow cytometry analysis was done for MSCs cell surface markers. MRC-5 subconfluent cells were starved with the medium containing 0. 5 % FBS for 24h, then treated with exogenous TGF-β 1 (10ng/ml as positive control) and MSCs-conditioned medium for 48h. Finally, the mRNA expression of three target genes: Collagen I, Collagen III and α-SMA were evaluated by RT-PCR technique. Results: Our findings demonstrated that bone marrow-derived mesenchymal stem cells-conditioned medium (secretome) significantly upregulated type I and III collagen expression but non-significantly α-SMA gene expression. Conclusion: Totally, Real Time PCR results suggest that MSCs conditioned medium activates differentiation of Fibroblast to myoFibroblast phenotype as confirmed through the presence of α-SMA, Collagen I and Collagen III expression compared to control in MRC 5 cells.

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    APA: Copy

    Jalili Angourani, Khadijeh, Mazhari, Sogol, FARIVAR, SHIRIN, Salman Mahini, Donya, Rouintan, Abdolreza, & BAGHAEI, KAVEH. (2018). Fibroblast-myofibroblast crosstalk after exposure to mesenchymal stem cells secretome. GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH, 11(suppl 1), 73-79. SID. https://sid.ir/paper/324809/en

    Vancouver: Copy

    Jalili Angourani Khadijeh, Mazhari Sogol, FARIVAR SHIRIN, Salman Mahini Donya, Rouintan Abdolreza, BAGHAEI KAVEH. Fibroblast-myofibroblast crosstalk after exposure to mesenchymal stem cells secretome. GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH[Internet]. 2018;11(suppl 1):73-79. Available from: https://sid.ir/paper/324809/en

    IEEE: Copy

    Khadijeh Jalili Angourani, Sogol Mazhari, SHIRIN FARIVAR, Donya Salman Mahini, Abdolreza Rouintan, and KAVEH BAGHAEI, “Fibroblast-myofibroblast crosstalk after exposure to mesenchymal stem cells secretome,” GASTROENTEROLOGY AND HEPATOLOGY FROM BED TO BENCH, vol. 11, no. suppl 1, pp. 73–79, 2018, [Online]. Available: https://sid.ir/paper/324809/en

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