MURINE MESENCHYMAL STEM CELL ISOLATION: IMPACT OF CELL-SEEDING DENSITY ON MORPHOLOGY, DIFFERENTIATION AND PROFILE OF SURFACE ANTIGENS

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BAGHBAN ESLAMINEZHAD M.R.; NADRI SAMAD

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Journal: KOWSAR MEDICAL JOURNAL Year:2008 | Volume:13 | Issue:1 Page(s): 37-49

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Title

MURINE MESENCHYMAL STEM CELL ISOLATION: IMPACT OF CELL-SEEDING DENSITY ON MORPHOLOGY, DIFFERENTIATION AND PROFILE OF SURFACE ANTIGENS

Author(s)

BAGHBAN ESLAMINEZHAD M.R. | NADRI SAMAD | Issue Writer Certificate

Keywords

MURINE MESENCHYMAL STEM CELLSQ3

CELL SEEDING DENSITYQ2

DIFFERENTIATIONQ3

SURFACE ANTIGENSQ3

Abstract

Objectives: The aim of the present study was to isolate MSCs using either low or high cell-seeding density culture system and to compare the cells produced in two systems in terms of their morphology, DIFFERENTIATION potential as well as the expression of some cell SURFACE ANTIGENS. Materials and Methods: The bone marrow cells from Balb/c mice of 6-8-weeks old were cultivated at 2.5×106 cells/cm2. Primary culture was then tripsinized, cultured either at 50 cells/cm2 as low density culture systems or at 8×104 cell/cm2 as high density culture system. The passaged-3 cells from either system were further examined and compared in terms of morphology, DIFFERENTIATION potential and the expression of some SURFACE ANTIGENS including CD135, CD44, CD31, Thy1.2, CD11b, CD45, CD34, Vcam1, Sca-1, and c-Kit. Results: In contrast to high–density culture system, in low density one, a few colons were produced and the culture contained more fibroblastic cells. According to the DIFFERENTIATION results, in contrast to high density system, more percentage of the cells being produced in low density culture system has been differentiated into bone and adipose cells. Furthermore, CD135, CD34, CD31 and Vcam were not appeared on the cells of low density system and Thy 1.2 surface antigen was not expressed on the cells of high density culture system. All these differences were statistically significant. Conclusions: Taken together, it seems that low density culture system is an appropriate method to isolate and expand MURINE MESENCHYMAL STEM CELLS.

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APA: Copy

BAGHBAN ESLAMINEZHAD, M.R., & NADRI, SAMAD. (2008). MURINE MESENCHYMAL STEM CELL ISOLATION: IMPACT OF CELL-SEEDING DENSITY ON MORPHOLOGY, DIFFERENTIATION AND PROFILE OF SURFACE ANTIGENS. KOWSAR MEDICAL JOURNAL, 13(1), 37-49. SID. https://sid.ir/paper/32688/en

Vancouver: Copy

BAGHBAN ESLAMINEZHAD M.R., NADRI SAMAD. MURINE MESENCHYMAL STEM CELL ISOLATION: IMPACT OF CELL-SEEDING DENSITY ON MORPHOLOGY, DIFFERENTIATION AND PROFILE OF SURFACE ANTIGENS. KOWSAR MEDICAL JOURNAL[Internet]. 2008;13(1):37-49. Available from: https://sid.ir/paper/32688/en

IEEE: Copy

M.R. BAGHBAN ESLAMINEZHAD, and SAMAD NADRI, “MURINE MESENCHYMAL STEM CELL ISOLATION: IMPACT OF CELL-SEEDING DENSITY ON MORPHOLOGY, DIFFERENTIATION AND PROFILE OF SURFACE ANTIGENS,” KOWSAR MEDICAL JOURNAL, vol. 13, no. 1, pp. 37–49, 2008, [Online]. Available: https://sid.ir/paper/32688/en

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