KINETIC PROPERTIES OF EXTRACTED CREATINE KINASE FROM MOUSE EMBRYONIC STEM CELLS-AND NEONATAL-DERIVED CARDIOMYOCYTES

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ZONOOZI R.; KAZEMI ASHTIANI S.; HOSSEINKHANI S.; BAHARVAND H.

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Journal: KOWSAR MEDICAL JOURNAL Year:2006 | Volume:11 | Issue:1 Page(s): 51-58

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Title

KINETIC PROPERTIES OF EXTRACTED CREATINE KINASE FROM MOUSE EMBRYONIC STEM CELLS-AND NEONATAL-DERIVED CARDIOMYOCYTES

Author(s)

ZONOOZI R. | KAZEMI ASHTIANI S. | HOSSEINKHANI S. | BAHARVAND H. | Issue Writer Certificate

Keywords

CREATINE KINASEQ3

KINETICQ3

MOUSE EMBRYONIC STEM CELLSQ3

MOUSE CARDIOMYOCYTESQ3

Abstract

Objective: Assessment of Metabolic and developmental condition of MOUSE EMBRYONIC STEM CELLS (ESC)-derived cardiomyocytes in Mice based on KINETIC properties of CREATINE KINASE (CK) compare with neonatal cardiomyocytes.Materials and Methods: ESCs were differentiated into beating cardiomyocytes spontaneously, and neonatal cardiomyocytes were isolated from 2-4 day old pulp mice.Total protein were extracted by sonication and centrifugation for determining KINETIC properties of CREATINE KINASE with spectrophotometer methods.Result: Specific activity of CK for CrP was 3.26±0.26 and 5.67±0.35 mM and its km was 50.25±2.21 and 43.48±2.04µmol min-1mg-1 in ESC-and neonatal-derived cardiomyocytes respectively. Optimum pH of CK activity was 7 for ESC-derived cardiomyocytes and 6.5 for neonatal-derived cardiomyocytes. The optimum temperature of CK activity was 45°C for both enzyme preparations.Conclusion: This data showed ESC-derived cardiomyocytes displayed a model that seems to resemble the in vitro development of cardiomyocytes.

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APA: Copy

ZONOOZI, R., KAZEMI ASHTIANI, S., HOSSEINKHANI, S., & BAHARVAND, H.. (2006). KINETIC PROPERTIES OF EXTRACTED CREATINE KINASE FROM MOUSE EMBRYONIC STEM CELLS-AND NEONATAL-DERIVED CARDIOMYOCYTES. KOWSAR MEDICAL JOURNAL, 11(1), 51-58. SID. https://sid.ir/paper/32784/en

Vancouver: Copy

ZONOOZI R., KAZEMI ASHTIANI S., HOSSEINKHANI S., BAHARVAND H.. KINETIC PROPERTIES OF EXTRACTED CREATINE KINASE FROM MOUSE EMBRYONIC STEM CELLS-AND NEONATAL-DERIVED CARDIOMYOCYTES. KOWSAR MEDICAL JOURNAL[Internet]. 2006;11(1):51-58. Available from: https://sid.ir/paper/32784/en

IEEE: Copy

R. ZONOOZI, S. KAZEMI ASHTIANI, S. HOSSEINKHANI, and H. BAHARVAND, “KINETIC PROPERTIES OF EXTRACTED CREATINE KINASE FROM MOUSE EMBRYONIC STEM CELLS-AND NEONATAL-DERIVED CARDIOMYOCYTES,” KOWSAR MEDICAL JOURNAL, vol. 11, no. 1, pp. 51–58, 2006, [Online]. Available: https://sid.ir/paper/32784/en

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