THE STUDY OF EXTRACELLULAR MATRIX AND CELL SURFACE GLYCOCONJUGATE IN RETINAL EMBRYONIC AND POSTNATAL DEVELOPMENT

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ARAB M.R.; TALAII T.; FAZEL A.R.

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Journal: KOWSAR MEDICAL JOURNAL Year:2000 | Volume:5 | Issue:Part 3 Page(s): 199-206

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Title

THE STUDY OF EXTRACELLULAR MATRIX AND CELL SURFACE GLYCOCONJUGATE IN RETINAL EMBRYONIC AND POSTNATAL DEVELOPMENT

Author(s)

ARAB M.R. | TALAII T. | FAZEL A.R. | Issue Writer Certificate

Keywords

RETINAQ3

EXTRACELLULAR MATRIXQ3

GLYCOCONJUGATEQ4

LECTINQ4

Abstract

The importance of EXTRACELLULAR MATRIX (ECM) and cell surface GLYCOCONJUGATE have been emphesized in tissue differentiation, cellular migration, epithelial fusion and cellular polarity. RETINA is a favourable model for cellular interactions and tissue differentiation. The aim of the present study was to investigate the role of ECM and GLYCOCONJUGATE in tissue differentiation. For these purposes, 50 Wistar rats were chosen and vaginal plug recognized the day 0 of gestation. Embryos from day 11 to 20 of gestation and newborns from day 1 to 15 were collected. Tissue blocks sectioned serially (5-6µm) and histochemical staining (H-E, Alcian blue, pH=1 and pH=2.5, PAS, Alcian blue pH=5.8 by critical electrolyte concentration method with four different concentration of magnesium) and LECTIN histochemistry via PNA and sialidase digestion/ PNA method were carried out. The results showed the presence of Gal/GalNac and sialic acid in optical surfaces of RPE and developing photoreceptors. Statistical analysis showed significant differences between embryonic and postnatal days for both hyalorunic acid (P < 0.05) and neutral saccharides (P<0.05, P<0.01). It seems that secretion of ECM and cell surface GLYCOCONJUGATE follow a spatiotemporal pattern and show the morphological events of organogenesis.

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مطالعات هیستوشیمیایی ترکیبات قندی سطح سلولی لنفوسیت های T در روند تکامل جنینی

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APA: Copy

ARAB, M.R., TALAII, T., & FAZEL, A.R.. (2000). THE STUDY OF EXTRACELLULAR MATRIX AND CELL SURFACE GLYCOCONJUGATE IN RETINAL EMBRYONIC AND POSTNATAL DEVELOPMENT. KOWSAR MEDICAL JOURNAL, 5(Part 3), 199-206. SID. https://sid.ir/paper/32789/en

Vancouver: Copy

ARAB M.R., TALAII T., FAZEL A.R.. THE STUDY OF EXTRACELLULAR MATRIX AND CELL SURFACE GLYCOCONJUGATE IN RETINAL EMBRYONIC AND POSTNATAL DEVELOPMENT. KOWSAR MEDICAL JOURNAL[Internet]. 2000;5(Part 3):199-206. Available from: https://sid.ir/paper/32789/en

IEEE: Copy

M.R. ARAB, T. TALAII, and A.R. FAZEL, “THE STUDY OF EXTRACELLULAR MATRIX AND CELL SURFACE GLYCOCONJUGATE IN RETINAL EMBRYONIC AND POSTNATAL DEVELOPMENT,” KOWSAR MEDICAL JOURNAL, vol. 5, no. Part 3, pp. 199–206, 2000, [Online]. Available: https://sid.ir/paper/32789/en

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