IMPROVED ALIGNMENT OF BISULFITE SEQUENCING DATA USING CPG ISLANDS

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BARJASTE NADIA; NADIMI REZA; ALIPOUR MAJID

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Journal Paper

Paper Information

Journal: JOURNAL OF ADVANCES IN COMPUTER RESEARCH Year:2014 | Volume:5 | Issue:2 (16) Page(s): 65-69

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Information Journal Paper

Title

IMPROVED ALIGNMENT OF BISULFITE SEQUENCING DATA USING CPG ISLANDS

Author(s)

BARJASTE NADIA | NADIMI REZA | ALIPOUR MAJID | Issue Writer Certificate

Keywords

BISULFITE SEQUENCING

DNA METHYLATION

ALIGNMENT TOOLS

Abstract

DNA methylation is an important biological process involving in human disease such as cancer Insomia and Diabetes. BISULFITE SEQUENCING (BS-Seq) with next-generation technology is an accurate method for measuring DNA METHYLATION. BS-seq data analysis is a considerable way to recognize methylated cytosines and several tools have been developed to analyze BS-Seq such as BS-Seeker, B-SOLANA, BRAT, BSMAP and etc. In this paper, we propose a novel idea to get more efficiency in the sequencing process, This idea will improve the rate of accuracy in the BSOLANA alignment tool using a new method in the preprocessing step. Our method is based on modification in some regions of DNA strand named CpG islands. CpG islands are significant regions in DNA strand which frequency of methylated cytosines is less than other CpG contexts. We compared our method with previous methods in the preprocessing of the original BSOLANA tool using on HG19 reads. The comparison shows that the new method provides more ability to align read sequences in the BSOLANA.

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APA: Copy

BARJASTE, NADIA, NADIMI, REZA, & ALIPOUR, MAJID. (2014). IMPROVED ALIGNMENT OF BISULFITE SEQUENCING DATA USING CPG ISLANDS. JOURNAL OF ADVANCES IN COMPUTER RESEARCH, 5(2 (16)), 65-69. SID. https://sid.ir/paper/328757/en

Vancouver: Copy

BARJASTE NADIA, NADIMI REZA, ALIPOUR MAJID. IMPROVED ALIGNMENT OF BISULFITE SEQUENCING DATA USING CPG ISLANDS. JOURNAL OF ADVANCES IN COMPUTER RESEARCH[Internet]. 2014;5(2 (16)):65-69. Available from: https://sid.ir/paper/328757/en

IEEE: Copy

NADIA BARJASTE, REZA NADIMI, and MAJID ALIPOUR, “IMPROVED ALIGNMENT OF BISULFITE SEQUENCING DATA USING CPG ISLANDS,” JOURNAL OF ADVANCES IN COMPUTER RESEARCH, vol. 5, no. 2 (16), pp. 65–69, 2014, [Online]. Available: https://sid.ir/paper/328757/en

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