CULTIVATION OF PNEUMOCYSTIS CARINII ON CELL LINES

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MOROVATI H.; MAHMOUDZADEH POURNAKI ABAS; PEYKARI H.; KAMALZADEH M.; LOTFI M.; MALDJAEI S.V.; HAJIZADEH A.; SHAHRAMYAR Z.

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Journal: KOWSAR MEDICAL JOURNAL Year:2006 | Volume:10 | Issue:4 Page(s): 263-270

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Information Journal Paper

Title

CULTIVATION OF PNEUMOCYSTIS CARINII ON CELL LINES

Author(s)

Keywords

PNEUMOCYSTIS CARINIIQ3

ISOLATIONQ3

CULTUREQ3

PROPAGATIONQ3

PRESERVATIONQ4

VEROQ4

MRC-5 AND RBK CELL LINESQ4

Abstract

Objective: Cultivation of PNEUMOCYSTIS CARINII on VERO andMRC-5cell lines and use of RBK Cell in Iran Materials and Methods: Six Sprague-Dawley rats obtained from Razi Vaccine Institute were immunosuppressed using methylprednisolone 40 mg/kg subcutaneously, weekly, for 8 weeks. The animals were maintained under near-sterile conditions to prevent secondary bacterial and fungal infection. After sacrifice, the lungs were removed and homogenate was prepared. Then relative purification of trophozoite occurred. The tissue CULTURE flasks were inoculated with trophozoite. Results: Five days after incubation 3, 3 and 3.75 fold respectively, increased in VERO, MRC-5 and PBK. Conclusion: This study can be a starting point for developing an extraction, cultivation and purification method of PNEUMOCYSTIS CARINII in Iran.

Cites

HISTOPATHOLOGY OF PNEUMOCYSTIS CARINII IN THE LUNG OF IMMUNODEFICIENT RATS

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APA: Copy

MOROVATI, H., MAHMOUDZADEH POURNAKI, ABAS, PEYKARI, H., KAMALZADEH, M., LOTFI, M., MALDJAEI, S.V., HAJIZADEH, A., & SHAHRAMYAR, Z.. (2006). CULTIVATION OF PNEUMOCYSTIS CARINII ON CELL LINES. KOWSAR MEDICAL JOURNAL, 10(4), 263-270. SID. https://sid.ir/paper/32915/en

Vancouver: Copy

MOROVATI H., MAHMOUDZADEH POURNAKI ABAS, PEYKARI H., KAMALZADEH M., LOTFI M., MALDJAEI S.V., HAJIZADEH A., SHAHRAMYAR Z.. CULTIVATION OF PNEUMOCYSTIS CARINII ON CELL LINES. KOWSAR MEDICAL JOURNAL[Internet]. 2006;10(4):263-270. Available from: https://sid.ir/paper/32915/en

IEEE: Copy

H. MOROVATI, ABAS MAHMOUDZADEH POURNAKI, H. PEYKARI, M. KAMALZADEH, M. LOTFI, S.V. MALDJAEI, A. HAJIZADEH, and Z. SHAHRAMYAR, “CULTIVATION OF PNEUMOCYSTIS CARINII ON CELL LINES,” KOWSAR MEDICAL JOURNAL, vol. 10, no. 4, pp. 263–270, 2006, [Online]. Available: https://sid.ir/paper/32915/en

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