STUDY OF LASER EFFECT ON APOPTOSIS INDUCTION IN THE ALVEOLAR MACROPHAGES

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SADRI M.; RABBANI O.; KOHIYAN A.

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Journal: KOWSAR MEDICAL JOURNAL Year:2006 | Volume:11 | Issue:2 Page(s): 143-154

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Title

STUDY OF LASER EFFECT ON APOPTOSIS INDUCTION IN THE ALVEOLAR MACROPHAGES

Author(s)

SADRI M. | RABBANI O. | KOHIYAN A. | Issue Writer Certificate

Keywords

HE-NE (LASER)Q3

APOPTOSISQ3

DNA FRAGMENTATION MACROPHAGEQ2

Abstract

Objective: In view of therapeutic applications of laser beam, this study determines the quality and quantity of laser. Effect on rat alveolar macrophages so after radiation in different doses the cells were in combated and their viability was measured. Then we investigated the APOPTOSIS induction, and sound out that this energy can affect then and consequently the immune system, depending on the does of vitiating. Material and Methods: In this study, alveolar macrophages were prepared by lavage and then treated with HE-NE (LASER) (wave length of 632 nm and power of 2Mw) in different phases. Time period of 3, 7, 12, 20 and 30minutes provided the following joules of energy: 0.36, 0.48, 1.8, 2.4 and 3.6 respectively. The samples were radiated on experimental suspensions containing 2 million cells per sample. The effect on the cell survival was studied and DNA fragmentation was measured using diphenylamine and agarose gel procedures after radiation and cell growth at standard conditions. Results: It is observed that the percent of cell survival increased up to 7 minutes radiation whereas it decreased significantly in exposure beyond 12 minutes. The percentage of DNA fragmentation increased beyond 10 minutes, and agarose gel electrophoresis confirmed the above observations. Cytological studies showed the morphologic changes in samples beyond 10 minutes exposure of laser radiation. Protein synthesis was increased in the samples undergoing 7 minutes exposure which was attributed to APOPTOSIS induction.Conclusion: The results showed that the effect of laser on the macrophage cells depends on the levels of energy absorbed based on the time of radiation. These observations suggest that the radiations beyond 10 minutes induce the APOPTOSIS process. In longer radiations necrosis occurs.

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APA: Copy

SADRI, M., RABBANI, O., & KOHIYAN, A.. (2006). STUDY OF LASER EFFECT ON APOPTOSIS INDUCTION IN THE ALVEOLAR MACROPHAGES. KOWSAR MEDICAL JOURNAL, 11(2), 143-154. SID. https://sid.ir/paper/33030/en

Vancouver: Copy

SADRI M., RABBANI O., KOHIYAN A.. STUDY OF LASER EFFECT ON APOPTOSIS INDUCTION IN THE ALVEOLAR MACROPHAGES. KOWSAR MEDICAL JOURNAL[Internet]. 2006;11(2):143-154. Available from: https://sid.ir/paper/33030/en

IEEE: Copy

M. SADRI, O. RABBANI, and A. KOHIYAN, “STUDY OF LASER EFFECT ON APOPTOSIS INDUCTION IN THE ALVEOLAR MACROPHAGES,” KOWSAR MEDICAL JOURNAL, vol. 11, no. 2, pp. 143–154, 2006, [Online]. Available: https://sid.ir/paper/33030/en

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