REVERSE TRANSCRIPTION-PCR ASSAY FOR TYPING AND SUB TYPING OF INFLUENZA A VIRUSES (H7 SUBTYPE)

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SABERFAR E.; FORGHANIFARD M.; NAJAFI A.; MOUSAVI M.

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Paper Information

Journal: KOWSAR MEDICAL JOURNAL Year:2006 | Volume:11 | Issue:2 Page(s): 119-124

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Title

REVERSE TRANSCRIPTION-PCR ASSAY FOR TYPING AND SUB TYPING OF INFLUENZA A VIRUSES (H7 SUBTYPE)

Author(s)

SABERFAR E. | FORGHANIFARD M. | NAJAFI A. | MOUSAVI M. | Issue Writer Certificate

Keywords

INFLUENZAQ2

HAEMAGGLUTININQ3

RT-PCRQ3

H7Q3

SUB TYPINGQ3

Abstract

Objective: Optimization of Reverse transcription-PCR assay for typing and SUB TYPING of INFLUENZA a virus (H7 subtype). Material and Methods: A RT-PCR was optimized on 6 standard INFLUENZA virus type A, using primers for the detection of INFLUENZA a virus and the H7 subtype. The INFLUENZA type A specific primers were directed to region of the INFLUENZA a matrix (M) gene that conserved among most type A INFLUENZA viruses. The H7 primers are directed to H7 HAEMAGGLUTININ (HA) gene regions that are conserved in H7 subtype. The selected primer sets were used in RT-PCR for type A. Results: The HA primer (H7F, H7R) for the detection of H7 INFLUENZA A subtypes amplified a band of 98 bp. A sharp band of the expected size was obtained from H7 strains tested and no PCR product was amplified from non-H7 INFLUENZA subtypes. Conclusions: Our results suggest that RT-PCR assay can be a useful test for detecting INFLUENZA A viruses and SUB TYPING of INFLUENZA H7 subtype.

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APA: Copy

SABERFAR, E., FORGHANIFARD, M., NAJAFI, A., & MOUSAVI, M.. (2006). REVERSE TRANSCRIPTION-PCR ASSAY FOR TYPING AND SUB TYPING OF INFLUENZA A VIRUSES (H7 SUBTYPE). KOWSAR MEDICAL JOURNAL, 11(2), 119-124. SID. https://sid.ir/paper/33036/en

Vancouver: Copy

SABERFAR E., FORGHANIFARD M., NAJAFI A., MOUSAVI M.. REVERSE TRANSCRIPTION-PCR ASSAY FOR TYPING AND SUB TYPING OF INFLUENZA A VIRUSES (H7 SUBTYPE). KOWSAR MEDICAL JOURNAL[Internet]. 2006;11(2):119-124. Available from: https://sid.ir/paper/33036/en

IEEE: Copy

E. SABERFAR, M. FORGHANIFARD, A. NAJAFI, and M. MOUSAVI, “REVERSE TRANSCRIPTION-PCR ASSAY FOR TYPING AND SUB TYPING OF INFLUENZA A VIRUSES (H7 SUBTYPE),” KOWSAR MEDICAL JOURNAL, vol. 11, no. 2, pp. 119–124, 2006, [Online]. Available: https://sid.ir/paper/33036/en

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