مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

294
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

193
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

PROTECTIVE EFFECT OF NAC AGAINST MALATHION-INDUCED OXIDATIVE STRESS IN FRESHLY ISOLATED RAT HEPATOCYTES

Pages

  79-88

Abstract

 Purpose: Induction of OXIDATIVE STRESS by Organophosphate compounds (OPs) has been previously reported. In the present work, the mechanism of protective effects of N-acetylcysteine as a glutathion (GSH) prodrug against malathion–induced cell toxicity was investigated. In this work, freshly isolated rat HEPATOCYTES were used to determine the effect of NAC on malathion-induced CYTOTOXICITY, formation of reactive oxygen species (ROS) and MITOCHONDRIAl dysfunction.Methods: Rat HEPATOCYTES were isolated using collagenase perfusion and then cell viability, mitchondrial membrane potential (MMP) and ROS formation were determined using trypan blue exclusion, Rhodamine 123 fluorescence and fluorogenic probe, 2’, 7’ -dichlorofluorescin diacetate (DCFH-DA), respectively.Results: Despite the protective effect of NAC on malathion-induced cell toxicity and MMP dysfunction, its efficacy against ROS formation was not adequate to completely protect the cells.Conclusion: Cytotoxic effects of malathion regardless of its cholinergic feature, is started with gradual free radical production but, the main factor that causes cell death, is MITOCHONDRIAl dysfunction, so that reduction of ROS formation alone is not sufficient for cell survival, and the maintenance of MITOCHONDRIAl integrity through different mechanisms is the most ameliorative factor specially at high levels of cell damage, as NAC seemed to protect cells with various fashions apart from ROS scavenging in concentrations higher than malathion’s LC50.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    MOSTAFALOU, SARA, ABDOLLAHI, MOHAMMAD, EGHBAL, MOHAMMAD ALI, & SAEEDI KOUZEHKONANI, NAZLI. (2012). PROTECTIVE EFFECT OF NAC AGAINST MALATHION-INDUCED OXIDATIVE STRESS IN FRESHLY ISOLATED RAT HEPATOCYTES. ADVANCED PHARMACEUTICAL BULLETIN, 2(1), 79-88. SID. https://sid.ir/paper/331712/en

    Vancouver: Copy

    MOSTAFALOU SARA, ABDOLLAHI MOHAMMAD, EGHBAL MOHAMMAD ALI, SAEEDI KOUZEHKONANI NAZLI. PROTECTIVE EFFECT OF NAC AGAINST MALATHION-INDUCED OXIDATIVE STRESS IN FRESHLY ISOLATED RAT HEPATOCYTES. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2012;2(1):79-88. Available from: https://sid.ir/paper/331712/en

    IEEE: Copy

    SARA MOSTAFALOU, MOHAMMAD ABDOLLAHI, MOHAMMAD ALI EGHBAL, and NAZLI SAEEDI KOUZEHKONANI, “PROTECTIVE EFFECT OF NAC AGAINST MALATHION-INDUCED OXIDATIVE STRESS IN FRESHLY ISOLATED RAT HEPATOCYTES,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 2, no. 1, pp. 79–88, 2012, [Online]. Available: https://sid.ir/paper/331712/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button