IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF GENES CODING PHARMACEUTICALLY IMPORTANT ENZYMES FROM HALO-THERMO TOLERANT BACILLUS

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SAFARY AZAM; MONIRI REZVAN; HAMZEH MIVEHROUD MARYAM; DASTMALCHI SIAVOUSH

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Journal: Advanced Pharmaceutical Bulletin Year:2016 | Volume:6 | Issue:4 Page(s): 551-561

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Title

IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF GENES CODING PHARMACEUTICALLY IMPORTANT ENZYMES FROM HALO-THERMO TOLERANT BACILLUS

Author(s)

SAFARY AZAM | MONIRI REZVAN | HAMZEH MIVEHROUD MARYAM | DASTMALCHI SIAVOUSH | Issue Writer Certificate

Keywords

MOLECULAR CHARACTERIZATION

PHARMACEUTICAL ENZYMES

GENE SEQUENCE

HALO-THERMO TOLERANT BACILLUS

Abstract

Purpose: Robust pharmaceutical and industrial enzymes from extremophile microorganisms are main source of enzymes with tremendous stability under harsh conditions which make them potential tools for commercial and biotechnological applications.Methods: The genome of a Gram-positive halo-thermotolerant Bacillus sp. SL1, new isolate from Saline Lake, was investigated for the presence of genes coding for potentially PHARMACEUTICAL ENZYMES. We determined GENE SEQUENCEs for the enzymes laccase (CotA), l-asparaginase (ansA3, ansA1), glutamate-specific endopeptidase (blaSE), l-arabinose isomerase (araA2), endo-1, 4-b mannosidase (gmuG), glutaminase (glsA), pectate lyase (pelA), cellulase (bglC1), aldehyde dehydrogenase (ycbD) and allantoinases (pucH) in the genome of Bacillus sp. SL1.Results: Based on the DNA sequence alignment results, six of the studied enzymes of Bacillus sp. SL-1 showed 100% similarity at the nucleotide level to the same genes of B. licheniformis 14580 demonstrating extensive organizational relationship between these two strains. Despite high similarities between the B. licheniformis and Bacillus sp. SL-1 genomes, there are minor differences in the sequences of some enzyme. Approximately 30% of the enzyme sequences revealed more than 99% identity with some variations in nucleotides leading to amino acid substitution in protein sequences.Conclusion: MOLECULAR CHARACTERIZATION of this new isolate provides useful information regarding evolutionary relationship between B. subtilis and B. licheniformis species. Since, the most industrial processes are often performed in harsh conditions, enzymes from such halo-thermotolerant bacteria may provide economically and industrially appealing biocatalysts to be used under specific physicochemical situations in medical, pharmaceutical, chemical and other industries.

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APA: Copy

SAFARY, AZAM, MONIRI, REZVAN, HAMZEH MIVEHROUD, MARYAM, & DASTMALCHI, SIAVOUSH. (2016). IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF GENES CODING PHARMACEUTICALLY IMPORTANT ENZYMES FROM HALO-THERMO TOLERANT BACILLUS. ADVANCED PHARMACEUTICAL BULLETIN, 6(4), 551-561. SID. https://sid.ir/paper/331846/en

Vancouver: Copy

SAFARY AZAM, MONIRI REZVAN, HAMZEH MIVEHROUD MARYAM, DASTMALCHI SIAVOUSH. IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF GENES CODING PHARMACEUTICALLY IMPORTANT ENZYMES FROM HALO-THERMO TOLERANT BACILLUS. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2016;6(4):551-561. Available from: https://sid.ir/paper/331846/en

IEEE: Copy

AZAM SAFARY, REZVAN MONIRI, MARYAM HAMZEH MIVEHROUD, and SIAVOUSH DASTMALCHI, “IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF GENES CODING PHARMACEUTICALLY IMPORTANT ENZYMES FROM HALO-THERMO TOLERANT BACILLUS,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 6, no. 4, pp. 551–561, 2016, [Online]. Available: https://sid.ir/paper/331846/en

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