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Information Journal Paper

Title

A CONVENIENT METHOD TO GENERATE METHYLATED AND UN-METHYLATED CONTROL DNA IN METHYLATION STUDIES

Pages

  57-61

Abstract

 Methylated and un-methylated CONTROL DNA is an important part of DNA METHYLATION studies. Although human and mouse DNA METHYLATION control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. In this paper a simple method of generating methylated and un-methylated CONTROL DNA is described. Whole genome amplification and enzymatic methylation were performed to generate un-methylated and methylated DNA.The generated DNA were confirmed using methylation sensitive/dependant enzymes, and methylation specific PCR. Control reaction assays confirmed the generated methylated and un-methylated DNA.

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    APA: Copy

    MANOOCHEHRI, MEHDI, BANDEHPOUR, MOJGAN, & KAZEMI, BAHRAM. (2013). A CONVENIENT METHOD TO GENERATE METHYLATED AND UN-METHYLATED CONTROL DNA IN METHYLATION STUDIES. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, 2(3), 57-61. SID. https://sid.ir/paper/335846/en

    Vancouver: Copy

    MANOOCHEHRI MEHDI, BANDEHPOUR MOJGAN, KAZEMI BAHRAM. A CONVENIENT METHOD TO GENERATE METHYLATED AND UN-METHYLATED CONTROL DNA IN METHYLATION STUDIES. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS[Internet]. 2013;2(3):57-61. Available from: https://sid.ir/paper/335846/en

    IEEE: Copy

    MEHDI MANOOCHEHRI, MOJGAN BANDEHPOUR, and BAHRAM KAZEMI, “A CONVENIENT METHOD TO GENERATE METHYLATED AND UN-METHYLATED CONTROL DNA IN METHYLATION STUDIES,” MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, vol. 2, no. 3, pp. 57–61, 2013, [Online]. Available: https://sid.ir/paper/335846/en

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