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Information Journal Paper

Title

A silk fibroin/decellularized extract of Wharton’ s jelly hydrogel intended for cartilage tissue engineering

Pages

  31-42

Abstract

 A hybrid hydrogel was obtained from decellularized extract from Wharton’; s jelly (DEWJ) and silk fibroin (SF) and characterized for Cartilage tissue engineering. Wharton’; s jelly was used due to its similarity with articular cartilage in extracellular matrix composition. Also, silk fibroin has good mechanical properties which make this construct appropriate for cartilage repair. Decellularization of Wharton’; s jelly was verified by DAPI staining, DNA quantification, and PCR analysis. Then, the biochemical composition of DEWJ was determined by ELISA kits for total proteins, collagens, sulfated glycosaminoglycans (sGAG), and transforming growth factor β 1 (TGF-β 1). After fabricating pure SF and SF/DEWJ hybrid hydrogels, their physical and mechanical properties were characterized by FESEM, Fourier-transform infrared spectroscopy (FTIR) and rheological assays (amplitude and frequency sweeps). Furthermore, cell viability and proliferation were assessed by MTT assay. The results have shown that DEWJ in hybrid hydrogels enhances mechanical properties of the construct relative to pure SF hydrogels. Also, this extract at its 40% concentration in culture media and 20% or 40% concentrations in SF/DEWJ hybrid hydrogels significantly increases population of the cells compared to control and pure SF hydrogel after 7 days. In conclusion, this study proposes the potential of SF/DEWJ hybrid hydrogels for Cartilage tissue engineering applications.

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    Cite

    APA: Copy

    Basiri, Arefeh, FAROKHI, MEHDI, Azami, Mahmoud, EBRAHIMI BAROUGH, SOMAYEH, MOHAMADNIA, ABDOLREZA, RASHTBAR, MORTEZA, Hasanzadeh, Elham, BAGHABAN ESLAMINEJAD, MOHAMADREZA, & AI, JAFAR. (2019). A silk fibroin/decellularized extract of Wharton’ s jelly hydrogel intended for cartilage tissue engineering. PROGRESS IN BIOMATERIALS, 8(1), 31-42. SID. https://sid.ir/paper/336547/en

    Vancouver: Copy

    Basiri Arefeh, FAROKHI MEHDI, Azami Mahmoud, EBRAHIMI BAROUGH SOMAYEH, MOHAMADNIA ABDOLREZA, RASHTBAR MORTEZA, Hasanzadeh Elham, BAGHABAN ESLAMINEJAD MOHAMADREZA, AI JAFAR. A silk fibroin/decellularized extract of Wharton’ s jelly hydrogel intended for cartilage tissue engineering. PROGRESS IN BIOMATERIALS[Internet]. 2019;8(1):31-42. Available from: https://sid.ir/paper/336547/en

    IEEE: Copy

    Arefeh Basiri, MEHDI FAROKHI, Mahmoud Azami, SOMAYEH EBRAHIMI BAROUGH, ABDOLREZA MOHAMADNIA, MORTEZA RASHTBAR, Elham Hasanzadeh, MOHAMADREZA BAGHABAN ESLAMINEJAD, and JAFAR AI, “A silk fibroin/decellularized extract of Wharton’ s jelly hydrogel intended for cartilage tissue engineering,” PROGRESS IN BIOMATERIALS, vol. 8, no. 1, pp. 31–42, 2019, [Online]. Available: https://sid.ir/paper/336547/en

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