PCR Identification of Escherichia coli Isolated from Bushehr Coastal Water

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

RAHIMI Z.; BAHADOR A.; AZIMZADEH M.; HAGHIGHI M.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian South Medical Journal Year:2018 | Volume:21 | Issue:5 Page(s): 383-392

Download Full-Text

Persian Verion

View:

769

Download:

Cites:

Information Journal Paper

Title

PCR Identification of Escherichia coli Isolated from Bushehr Coastal Water

Author(s)

RAHIMI Z. | BAHADOR A. | AZIMZADEH M. | HAGHIGHI M.A. | Issue Writer Certificate

Keywords

Escherichia coli.Indicator microorganismQ2

Molecular identificationQ1

Polymerase Chain ReactionQ1

Abstract

Background: Standard methods of identification have not been able to solve all issues concerning E. coli. With the development of genomic studies, PCR appears promising to deal with the shortcomings. This study aimed to utilize PCR with specific primers for lacZ, uidA, cyd, and lacY gene segments to identify environmental E. coli isolates. Materials and methods: PCR and the aforementioned four primers were used for Molecular identification of E. coli on purified genome DNA from 120 environmental E. coli isolates, standard strains of Shigella, and Enterohaemorrhagic E. coli strain as controls. All environmental E. coli isolates were isolated from Bushehr coastal areas and identified in a previous study by standard bacteriological methods and then preserved in-70 ˚ C for further studies. Results: The primers successfully showed their ability to identify the targets in environmental isolates and standard strains. It is shown that the four PCR fragments related to lacZ, uidA, cyd, and lacY genes were observed only for E. coli isolates and strains. Conclusion: PCR method proved capable to distinguish E. coli from Shigella as the most phylogenetically related genus and contrary to the classical methods, it could detect enterohaemorrhagic strains as Escherichia coli.

Cites

No record.

References

No record.

Cite

APA: Copy

RAHIMI, Z., BAHADOR, A., AZIMZADEH, M., & HAGHIGHI, M.A.. (2018). PCR Identification of Escherichia coli Isolated from Bushehr Coastal Water. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), 21(5 ), 383-392. SID. https://sid.ir/paper/33760/en

Vancouver: Copy

RAHIMI Z., BAHADOR A., AZIMZADEH M., HAGHIGHI M.A.. PCR Identification of Escherichia coli Isolated from Bushehr Coastal Water. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)[Internet]. 2018;21(5 ):383-392. Available from: https://sid.ir/paper/33760/en

IEEE: Copy

Z. RAHIMI, A. BAHADOR, M. AZIMZADEH, and M.A. HAGHIGHI, “PCR Identification of Escherichia coli Isolated from Bushehr Coastal Water,” IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), vol. 21, no. 5 , pp. 383–392, 2018, [Online]. Available: https://sid.ir/paper/33760/en

Related Journal Papers