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Cites:

1

Information Journal Paper

Title

Construction of a Novel DNA Vaccine Candidate encoding LmSTI1-PpSP42 Fusion Protein from Leishmania major and Phlebotomus papatasi Against Cutaneous Leishmaniasis

Pages

  67-67

Abstract

 Background: Cutaneous leishmaniasis (CL) is a serious public health problem in many tropical countries. The infection is caused by a protozoan parasite of Leishmania genus transmitted by Phlebotominae sandflies. In the present study, we constructed a eukaryotic expression vector to produce a fusion protein containing LmSTI1 from Leishmania major (L. major) and PpSP42 from Phlebotomus papatasi (Ph. papatasi). In future studies we will test this construct as a DNA vaccine against zoonotic CL. Methods: The nucleotide sequences encoding the LmSTI1 protein and a fragment encoding 79% of PpSP42 were amplified using L. major and Ph. papatasi genomic DNA, respectively. The amplicons were cloned into the pcDNA3. 1(+) eukaryotic expression vector. The recombinant plasmid pcDNA-LmSTI1Pp42 was propagated in Escherichia coli (E. coli) and used to transfect HEK-293T cells. The expressed fusion protein was analyzed by Western blotting using anti-LmSTI1 mouse serum. Results: Sequences encoding LmSTI1 and partial PpSP42 were cloned into pcDNA3. 1(+). Production of the recombinant LmSTI1Pp42 fusion protein was confirmed by Western blotting. Conclusions: An LmSTI1Pp42 fusion protein was expressed in HEK-293T cells. This construct may be an effective DNA vaccine against CL.

Cites

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    APA: Copy

    MIANDOABI, Touraj, Bahrami, Fariborz, MOEIN VAZIRI, Vahideh, & AJDARY, SOHEILA. (2018). Construction of a Novel DNA Vaccine Candidate encoding LmSTI1-PpSP42 Fusion Protein from Leishmania major and Phlebotomus papatasi Against Cutaneous Leishmaniasis. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 7(1), 67-67. SID. https://sid.ir/paper/343147/en

    Vancouver: Copy

    MIANDOABI Touraj, Bahrami Fariborz, MOEIN VAZIRI Vahideh, AJDARY SOHEILA. Construction of a Novel DNA Vaccine Candidate encoding LmSTI1-PpSP42 Fusion Protein from Leishmania major and Phlebotomus papatasi Against Cutaneous Leishmaniasis. REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY[Internet]. 2018;7(1):67-67. Available from: https://sid.ir/paper/343147/en

    IEEE: Copy

    Touraj MIANDOABI, Fariborz Bahrami, Vahideh MOEIN VAZIRI, and SOHEILA AJDARY, “Construction of a Novel DNA Vaccine Candidate encoding LmSTI1-PpSP42 Fusion Protein from Leishmania major and Phlebotomus papatasi Against Cutaneous Leishmaniasis,” REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 7, no. 1, pp. 67–67, 2018, [Online]. Available: https://sid.ir/paper/343147/en

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