CHROMOSOMAL STUDY ON ZAGROS PUPFISH (APHANIUS VLADYKOVI)

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AMINI F.; HEMATZADEH A.

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Journal Paper

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Journal: Journal of Veterinary Research Year:2012 | Volume:67 | Issue:3 Page(s): 291-296

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Information Journal Paper

Title

CHROMOSOMAL STUDY ON ZAGROS PUPFISH (APHANIUS VLADYKOVI)

Author(s)

AMINI F. | HEMATZADEH A. | Issue Writer Certificate

Keywords

APHANIUS VLADYKOVIQ2

CHROMOSOMEQ2

KARYOTYPEQ2

ZAGROS PUPFISHQ2

Abstract

BACKGROUND: ZAGROS PUPFISH (APHANIUS VLADYKOVI) is a native fish of Iran which is found in basins of Chahar Mahal & Bakhtiari province. OBJECTIVES: In this study, the CHROMOSOME number and KARYOTYPE of ZAGROS PUPFISH were investigated. METHODS: To obtain metaphase CHROMOSOME spreadsin vivo, 1.2-5.4 g fish were injected intraperitoneally by 0.1-0.15 mg/g of 1% colchicine and were humanely killed after being incubated in a well-aerated tank for 4-5 hours at 22-23oC. Hematopoietic, testis or ovary, gill, spleen and liver tissues were isolated from the fish and were fixed with cold Carnoy's solution after being hypotonized in 0.075 M KCl. CHROMOSOME spreads were prepared by either splashing of cell suspension or stamping of whole tissues onto slides. Slides were then stained by 10% Giemsa followed by microscopic observation. Suitable metaphase plates were digitally microphotographed and CHROMOSOMEs were counted and KARYOTYPEd. RESULTS: Diploid CHROMOSOME number ranged from 42 to 49 with the modal number (2n) of 48. Based on the prepared KARYOTYPE, CHROMOSOME formula for this species was 12st+36 a/t and the number of CHROMOSOME arms (FN) was calculated 60. No heteromorphic sex CHROMOSOMEs could be recognized in this species. CONCLUSIONS: The diploid CHROMOSOME number of ZAGROS PUPFISH is similar to other species of the same genus so far investigated.

Cites

C-, G-, NOR- AND RESTRICTION ENZYME- BANDING AND DAPI STAINING OF CHROMOSOMES OF ZAGROS PUPFISH APHANIUS VLADYKOVI

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APA: Copy

AMINI, F., & HEMATZADEH, A.. (2012). CHROMOSOMAL STUDY ON ZAGROS PUPFISH (APHANIUS VLADYKOVI). JOURNAL OF VETERINARY RESEARCH, 67(3), 291-296. SID. https://sid.ir/paper/34559/en

Vancouver: Copy

AMINI F., HEMATZADEH A.. CHROMOSOMAL STUDY ON ZAGROS PUPFISH (APHANIUS VLADYKOVI). JOURNAL OF VETERINARY RESEARCH[Internet]. 2012;67(3):291-296. Available from: https://sid.ir/paper/34559/en

IEEE: Copy

F. AMINI, and A. HEMATZADEH, “CHROMOSOMAL STUDY ON ZAGROS PUPFISH (APHANIUS VLADYKOVI),” JOURNAL OF VETERINARY RESEARCH, vol. 67, no. 3, pp. 291–296, 2012, [Online]. Available: https://sid.ir/paper/34559/en

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