Latex agglutination (LAT) and indirect haemagglutination (IHA) evaluation techniques to detect sheep dicrocoeliosis

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RAZI JALALI M.H.; GHORBANPOOR M.; Jahangiri nasr f.

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Journal: Journal of Veterinary Research Year:2018 | Volume:72 | Issue:4 Page(s): 391-396

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Title

Latex agglutination (LAT) and indirect haemagglutination (IHA) evaluation techniques to detect sheep dicrocoeliosis

Author(s)

RAZI JALALI M.H. | GHORBANPOOR M. | Jahangiri nasr f. | Issue Writer Certificate

Keywords

Dicrocoelium dendriticumQ2

Latex agglutinationQ1

Indirect haemagglutinationQ1

SheepQ2

Abstract

BACKGROUND: Dicrocoeliosis is caused by the small liver fluke Dicrocoelium dendriticum which lives in bile ducts and gall bladder of wild and domestic ruminant. Immunodiagnostic methods are useful for early diagnosis. OBJECTIVES: The purpose of this study was to evaluate agglutination latex (LAT) and Indirect haemagglutination (IHA) tests for diagnosis of Dicrocoelium dendriticum infection in Sheep. METHODS: Adult helminths were collected from infected livers of slaughtered Sheep at Khoramabad abattoir. The excretory-secretory and somatic antigens were provided using homogenization and sonication techniques. To provide positive and negative sera, blood was taken from infected and non-infected Sheep with Dicrocoelium dendriticum. Somatic and excretory-secretory antigens were added and blended to latex particles. All sera were added to latex and used for agglutination test. Sensitive RBC and somatic and ES antigens were added to IHA and blended to evaluate haemagglutination response. RESULTS: The sensitivity and specificity of LAT using excretory secretory and somatic antigens were 84%, 97/6%, 96% and 97/6% respectively. While the sensitivity and specificity in IHA using excretory secretory and somatic antigens were respectively 60%, 92/9%, 92% and 66/7%. CONCLUSIONS: From the results of the present study, the LAT had the highest sensitivity and specificity, which recommends it being considered as a rapid diagnostic technique for Sheep dicrocoeliosis.

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APA: Copy

RAZI JALALI, M.H., GHORBANPOOR, M., & Jahangiri nasr, f.. (2018). Latex agglutination (LAT) and indirect haemagglutination (IHA) evaluation techniques to detect sheep dicrocoeliosis. JOURNAL OF VETERINARY RESEARCH, 72(4 ), 391-396. SID. https://sid.ir/paper/34627/en

Vancouver: Copy

RAZI JALALI M.H., GHORBANPOOR M., Jahangiri nasr f.. Latex agglutination (LAT) and indirect haemagglutination (IHA) evaluation techniques to detect sheep dicrocoeliosis. JOURNAL OF VETERINARY RESEARCH[Internet]. 2018;72(4 ):391-396. Available from: https://sid.ir/paper/34627/en

IEEE: Copy

M.H. RAZI JALALI, M. GHORBANPOOR, and f. Jahangiri nasr, “Latex agglutination (LAT) and indirect haemagglutination (IHA) evaluation techniques to detect sheep dicrocoeliosis,” JOURNAL OF VETERINARY RESEARCH, vol. 72, no. 4 , pp. 391–396, 2018, [Online]. Available: https://sid.ir/paper/34627/en

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