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Information Journal Paper

Title

DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR

Pages

  11-16

Abstract

 In this study a nested-PCR assay was optimized for detection of two BVDV biotype of NADL strain. A part of 5' non-coding region of virus, 249 bp in size, was amplified in RT-PCR. PCR product was cloned in a pTZ57R/T vector and sequencing results confirmed the specificity of the test. Internal primers were designed and a 155 bp DNA fragment was amplified in nested-PCR. The sensitivity of RT-PCR and nested-PCR for detection of virus in cell culture were found to be 104 TCID50 and 102 TCID50, respectively. Seven cell cultures were tested for BVDV contamination using ELISA, RT-PCR and nested-PCR. Results indicate that sensitivity of molecular tests for detection of virus in cell culture samples is higher than ELISA.

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  • Cite

    APA: Copy

    GHOREYSHI, S.A., DALIRI JOUPARI, M., MORSHEDI, D., HAJIAN, T., & LOTFI, MOHSEN. (2008). DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR. JOURNAL OF VETERINARY RESEARCH, 63(1), 11-16. SID. https://sid.ir/paper/34768/en

    Vancouver: Copy

    GHOREYSHI S.A., DALIRI JOUPARI M., MORSHEDI D., HAJIAN T., LOTFI MOHSEN. DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR. JOURNAL OF VETERINARY RESEARCH[Internet]. 2008;63(1):11-16. Available from: https://sid.ir/paper/34768/en

    IEEE: Copy

    S.A. GHOREYSHI, M. DALIRI JOUPARI, D. MORSHEDI, T. HAJIAN, and MOHSEN LOTFI, “DETECTION OF PESTIVIRUS CONTAMINATION IN CELL CULTURES BY NESTED-PCR,” JOURNAL OF VETERINARY RESEARCH, vol. 63, no. 1, pp. 11–16, 2008, [Online]. Available: https://sid.ir/paper/34768/en

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