INTRAUTERINE LAPAROSCOPIC ARTIFICIAL INSEMINATION AND CERVICAL INSEMINATION IN SUPEROVULATED EWES FOR EMBRYO TRANSFER

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HOSSEINI PAZHOUH KH.; TAJIK PARVIZ; GHARAHGOUZLOU FARAMARZ

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Journal: Journal of Veterinary Research Year:2000 | Volume:55 | Issue:4 Page(s): 61-66

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Title

INTRAUTERINE LAPAROSCOPIC ARTIFICIAL INSEMINATION AND CERVICAL INSEMINATION IN SUPEROVULATED EWES FOR EMBRYO TRANSFER

Author(s)

HOSSEINI PAZHOUH KH. | TAJIK PARVIZ | GHARAHGOUZLOU FARAMARZ | Issue Writer Certificate

Keywords

INTRAUTERINEQ3

LAPAROSCOPYQ3

EWESQ2

EMBRYO TRANSFERQ4

Abstract

In order to study efficacy of cervical AI (once and twice inseminations) and laparoscopic INTRAUTERINE insemination, in egg recovery rate and fertilization rate, three experiments were done. In each of the 1st and 2nd experiments, 5 moghani EWES were inseminated in the external oss of cervix. EWES had been synchronized by vaginal sponges and superovulated by injections of 800 or 1200 IU of eCG. Insemination was done at 35-40 hours after sponge removal (the times of experiments and the EWES were different). In the first experiment, every ewe was inseminsted with 0.2 to 0.3 ml of semen diluted by skimed milk and in the second one, every ewe was inseminated with the same volume of semen diluted by tris buffer each contained 200-300´106 live motile sperm cells. The maximum interval between obtaining semen and insemination was 3 hours. In the first experiment, every ewe was injected 100 IU eCG immidiately after insemination. In the third experiment 8 Ghezel X Ciucy EWES were synchronized by PGF2a and superovulated by FSH, and divided into two groups. Bothgroups were inseminated by a semen diluted by tris-buffer and contained 100´106 sperm cells/ml. The first group was inseminated cervicaly with 0.3 ml of diluted semen twice, at 42 and 48 h after the last injection of FSH considering heat detection. In the 2nd group, laparoscopic INTRAUTERINE insemination was performed by 0.1 ml of diluted semen into each uterine horn at 44-45 h after the last injection of FSH. In bouth groups, 60 micro gram of GnRH was injected immidiatly after insemination (in the first group after the first insemination). Laparotomy was performed on all of the EWES 6 day after insemination, and uterine horns were flushed for recovery of the embryos. In the first experiment, 13 eggs were recovered from 23 ovulations (recovery rate=57%), of the 7 were fertilized (54%). In the 2nd experiment, 11 eggs were recovered in 35 ovulations (31% recovery rate) and even one egg was not fertilized (0% fertilization rate). In the first group among the 3rd experiment (twice cervical inseminations), 23 eggs were recovered from 41 ovulations (58% recovery rate) in which 14 were fertilized (65% fertilization rate) and 10 were suitable for EMBRYO TRANSFER. In the 2nd group of this experiment (laparoscopic insemination), 18 eggs were recovered from 23 ovulations (78% recovery rate) and all were fertilized (100%) in which 11 were suitable for EMBRYO TRANSFER. In conclusion these experiments indicate that cervical insemination may not be a suitable method in supervuiated EWES. In contrast, INTRAUTERINE insemination by LAPAROSCOPY is an effective method and even in comparision with twice cervical inseminations, it is significantly more effective. Correlation rate (Chooprov index) between insemination method and fertilization rate in the 3rd experiment was 47%.

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APA: Copy

HOSSEINI PAZHOUH, KH., TAJIK, PARVIZ, & GHARAHGOUZLOU, FARAMARZ. (2000). INTRAUTERINE LAPAROSCOPIC ARTIFICIAL INSEMINATION AND CERVICAL INSEMINATION IN SUPEROVULATED EWES FOR EMBRYO TRANSFER. JOURNAL OF VETERINARY RESEARCH, 55(4), 61-66. SID. https://sid.ir/paper/35201/en

Vancouver: Copy

HOSSEINI PAZHOUH KH., TAJIK PARVIZ, GHARAHGOUZLOU FARAMARZ. INTRAUTERINE LAPAROSCOPIC ARTIFICIAL INSEMINATION AND CERVICAL INSEMINATION IN SUPEROVULATED EWES FOR EMBRYO TRANSFER. JOURNAL OF VETERINARY RESEARCH[Internet]. 2000;55(4):61-66. Available from: https://sid.ir/paper/35201/en

IEEE: Copy

KH. HOSSEINI PAZHOUH, PARVIZ TAJIK, and FARAMARZ GHARAHGOUZLOU, “INTRAUTERINE LAPAROSCOPIC ARTIFICIAL INSEMINATION AND CERVICAL INSEMINATION IN SUPEROVULATED EWES FOR EMBRYO TRANSFER,” JOURNAL OF VETERINARY RESEARCH, vol. 55, no. 4, pp. 61–66, 2000, [Online]. Available: https://sid.ir/paper/35201/en

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