PREPARATION AND BIOCHEMICAL CHARACTERISATION OF NANOCONJUGATES OF FUNCTIONALIZED CARBON NANOTUBES AND CYTOCHROME C

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PATILA MICHAELA; DIAMANTI EVMORFIA K.; BERGOUNI DANAI; POLYDERA ANGELIKI C.; GOURNIS DIMITRIOS; STAMATIS HARALAMBOS

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Journal: NANOMEDICINE RESEARCH JOURNAL Year:2018 | Volume:3 | Issue:1 Page(s): 10-18

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Title

PREPARATION AND BIOCHEMICAL CHARACTERISATION OF NANOCONJUGATES OF FUNCTIONALIZED CARBON NANOTUBES AND CYTOCHROME C

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Keywords

BIOCATALYSIS

CARBON NANOTUBES

CYTOCHROME C

IMMOBILIZATION

NANOCONJUGATE

Abstract

Objective(s): The present work deals with the preparation of nanobioconjugates based on the IMMOBILIZATION of CYTOCHROME C (cyt c) on functionalized multiwall CARBON NANOTUBES (f-MWCNTs). The effect of the nanosupport and the IMMOBILIZATION procedure on the biochemical and structural characteristics of the immobilized protein was investigated.Methods: The MWCNTs were functionalized to provide alkyl chains with different length and terminal functional groups on their surface. The IMMOBILIZATION of cyt c was achieved through physical adsorption and covalent binding. Cyt-c-based NANOCONJUGATEs were characterized in terms of peroxidase activity and stability of protein, while UV-visible spectroscopy was used to investigate the structural characteristics of the immobilized protein.Results: The loading of cyt c on f-MWCNTs was effectively achieved, with IMMOBILIZATION yields reaching up to 77%. The peroxidase activity of cyt c was higher in the case of non covalent IMMOBILIZATION compared to that of covalent procedure. Immobilized cyt c exhibited higher thermal stability than the native protein after 24 h incubation at 40oC, while it preserved up to 100% of its initial activity after incubation in the presence of a denaturing agent such as H2O2. No significant changes in the heme microenvironment of cyt c were observed in the presence of f-MWCNTs.Conclusions: This study has demonstrated that f-MWCNTs are effective supports for the IMMOBILIZATION of cyt c, providing a universally applicable platform for the development of bionanoconjugates with potential use in a wide variety of fields in nanoBIOCATALYSIS, biosensing and nanomedicine.

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APA: Copy

PATILA, MICHAELA, DIAMANTI, EVMORFIA K., BERGOUNI, DANAI, POLYDERA, ANGELIKI C., GOURNIS, DIMITRIOS, & STAMATIS, HARALAMBOS. (2018). PREPARATION AND BIOCHEMICAL CHARACTERISATION OF NANOCONJUGATES OF FUNCTIONALIZED CARBON NANOTUBES AND CYTOCHROME C. NANOMEDICINE RESEARCH JOURNAL, 3(1), 10-18. SID. https://sid.ir/paper/352782/en

Vancouver: Copy

PATILA MICHAELA, DIAMANTI EVMORFIA K., BERGOUNI DANAI, POLYDERA ANGELIKI C., GOURNIS DIMITRIOS, STAMATIS HARALAMBOS. PREPARATION AND BIOCHEMICAL CHARACTERISATION OF NANOCONJUGATES OF FUNCTIONALIZED CARBON NANOTUBES AND CYTOCHROME C. NANOMEDICINE RESEARCH JOURNAL[Internet]. 2018;3(1):10-18. Available from: https://sid.ir/paper/352782/en

IEEE: Copy

MICHAELA PATILA, EVMORFIA K. DIAMANTI, DANAI BERGOUNI, ANGELIKI C. POLYDERA, DIMITRIOS GOURNIS, and HARALAMBOS STAMATIS, “PREPARATION AND BIOCHEMICAL CHARACTERISATION OF NANOCONJUGATES OF FUNCTIONALIZED CARBON NANOTUBES AND CYTOCHROME C,” NANOMEDICINE RESEARCH JOURNAL, vol. 3, no. 1, pp. 10–18, 2018, [Online]. Available: https://sid.ir/paper/352782/en

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