COMPARISON OF BONE CELL VIABILITY AND PROLIFERATION IN 3D SCAFFOLD TO MONOLAYER CELL CULTURE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AZIZI FAEZEH; Omidpanah Sahar; MORADI AFSHIN; HOSSINI MOHAMMAD ALI; ALIAKBARI FERESHTEH; SHARIATPANAHI SAMIRA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Archives of Men's Health Year:2018 | Volume:2 | Issue:1 Page(s): 0-0

Download Full-Text

View:

229

Download:

Cites:

Information Journal Paper

Title

COMPARISON OF BONE CELL VIABILITY AND PROLIFERATION IN 3D SCAFFOLD TO MONOLAYER CELL CULTURE

Author(s)

Keywords

ALGINATE

BONE GRAFT

MONOLAYER CULTURE

OSTEOBLAST

THREE- DIMENSIONAL SCAFFOLD

Abstract

Introduction: Today, due to high rates of accidents and fractures leading to bone defects and due to the limited possibility of BONE GRAFT bonding, using the patient’s cell culture on appropriate scaffolds and transferring it to the defect area is suggested as one of the treatment plans.Materials and methods: Bone samples of 8 male subjects that were under craniotomy surgery in the hospital were collected. First, the samples were cut into smaller pieces and then, transferred to incubator culture dishes. Two weeks later, the OSTEOBLAST activity on the bone matrix began and on average, the cells covered the dishes within two weeks. The first generation of the cells was re-moved by Trypsin_EDTA method from the opaltes, then were divided into two parts, one was added to ALGINATE gel and the other to MONOLAYER CULTURE. In order to prove the OSTEOBLAST activity on the bone matrix and investigate these activities, Van Kossa staining method was used, and also to investigate the cell viability, MTT method was employed.Results: There was a significant difference in the number of the cells created in ALGINATE gel and those created in monolayer after two weeks (P<0.001). Moreover, the difference between mean cell counts in ALGINATE gel and monolayer was statistically significant (P<0.001). The results of the MTT test in second week showed that the number of alive cells is significantly higher in ALGINATE gel (P<0.001). Finally, the result of the Van Kossa method proved extracellular matrix in both experimental groups.Conclusion: Results showed that ALGINATE gel better can support duplication and survival of OSTEOBLASTs compared to MONOLAYER CULTURE. This may be attributed to the biological properties of this gel; ALGINATE gel porosity provides conditions under which cellular and metabolic activities are accelerated.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

AZIZI, FAEZEH, Omidpanah, Sahar, MORADI, AFSHIN, HOSSINI, MOHAMMAD ALI, ALIAKBARI, FERESHTEH, & SHARIATPANAHI, SAMIRA. (2018). COMPARISON OF BONE CELL VIABILITY AND PROLIFERATION IN 3D SCAFFOLD TO MONOLAYER CELL CULTURE. MENS HEALTH JOURNAL, 2(1), 0-0. SID. https://sid.ir/paper/352891/en

Vancouver: Copy

AZIZI FAEZEH, Omidpanah Sahar, MORADI AFSHIN, HOSSINI MOHAMMAD ALI, ALIAKBARI FERESHTEH, SHARIATPANAHI SAMIRA. COMPARISON OF BONE CELL VIABILITY AND PROLIFERATION IN 3D SCAFFOLD TO MONOLAYER CELL CULTURE. MENS HEALTH JOURNAL[Internet]. 2018;2(1):0-0. Available from: https://sid.ir/paper/352891/en

IEEE: Copy

FAEZEH AZIZI, Sahar Omidpanah, AFSHIN MORADI, MOHAMMAD ALI HOSSINI, FERESHTEH ALIAKBARI, and SAMIRA SHARIATPANAHI, “COMPARISON OF BONE CELL VIABILITY AND PROLIFERATION IN 3D SCAFFOLD TO MONOLAYER CELL CULTURE,” MENS HEALTH JOURNAL, vol. 2, no. 1, pp. 0–0, 2018, [Online]. Available: https://sid.ir/paper/352891/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops