SEROLOGIC SURVEY OF CATTLE INFECTED WITH BVD VIRUS BY INDIRECT ELLSA AND COMPARE THE USE OF MILK - ELLSA AND SERUM - ELLSA IN URMIA

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MORSHEDI A.; MAHMOUDIAN A.L.; DALIR NAGHADEH B.; HAJIZADE J.

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Journal: Journal of Veterinary Research Year:2004 | Volume:59 | Issue:3 Page(s): 227-231

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Title

SEROLOGIC SURVEY OF CATTLE INFECTED WITH BVD VIRUS BY INDIRECT ELLSA AND COMPARE THE USE OF MILK - ELLSA AND SERUM - ELLSA IN URMIA

Author(s)

MORSHEDI A. | MAHMOUDIAN A.L. | DALIR NAGHADEH B. | HAJIZADE J. | Issue Writer Certificate

Keywords

MILK - ELISAQ4

BVDV SEROPREVALENCEQ4

CATTLEQ3

Abstract

Objective: Detecting of seropositive CATTLE to BVD virus by indirect ELISA using milk and serum samples from individual cows, comparing the use of milk instead of blood serum for determining the BVDV infection in CATTLE herds. Design: Bovine viral diarrhea seroprevalence survey in CATTLE using milk and serum ELISA by retrospective study. Animals: One hundred and eighty eight milky cows and 45 alves 0.5-2 years old (Native and hybride) from 24 CATTLE herds in subord of Urmia. Procedure: Preparing serum and skim milk for detection of antiBVDV antibody by indirect ELISA. The sera were diluted 1:25 and the milk samples were used as undiluted. The sera and milk which had OD equal or higher than 2.5 time OD of reference negative control serum and milk, considered as ELISA-positive. The data obtained from 188 matched sets of milk and serum were statistically compared with each other to determine the precentage of correlation between them. Statistical analysis: Student’s t and chi square tests. Reults: Out of 188 pairs milk and serum, 52 cases (27.65%) of milk and 59 (31.38%) of serum samples were ELISA positive. The data showed 96% correlation between the results of milk-EllS A and serum-ELISA. Conclusion: The present study showed that milk-ELISA is well performed in compared with serum-ELISA test. However the output of milk-EllS A was 3.72% less than serum-ELISA in detection of animals infected with BVDV, but on significant differences was found between the results gained by milk-ELISA (27.65%) and serum- ELISA (31.3 8%) test by student’s t -test. Therefore, the milk can be used instead of serum in ELISA for detection of infected herds as a screening test.

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APA: Copy

MORSHEDI, A., MAHMOUDIAN, A.L., DALIR NAGHADEH, B., & HAJIZADE, J.. (2004). SEROLOGIC SURVEY OF CATTLE INFECTED WITH BVD VIRUS BY INDIRECT ELLSA AND COMPARE THE USE OF MILK - ELLSA AND SERUM - ELLSA IN URMIA. JOURNAL OF VETERINARY RESEARCH, 59(3), 227-231. SID. https://sid.ir/paper/35334/en

Vancouver: Copy

MORSHEDI A., MAHMOUDIAN A.L., DALIR NAGHADEH B., HAJIZADE J.. SEROLOGIC SURVEY OF CATTLE INFECTED WITH BVD VIRUS BY INDIRECT ELLSA AND COMPARE THE USE OF MILK - ELLSA AND SERUM - ELLSA IN URMIA. JOURNAL OF VETERINARY RESEARCH[Internet]. 2004;59(3):227-231. Available from: https://sid.ir/paper/35334/en

IEEE: Copy

A. MORSHEDI, A.L. MAHMOUDIAN, B. DALIR NAGHADEH, and J. HAJIZADE, “SEROLOGIC SURVEY OF CATTLE INFECTED WITH BVD VIRUS BY INDIRECT ELLSA AND COMPARE THE USE OF MILK - ELLSA AND SERUM - ELLSA IN URMIA,” JOURNAL OF VETERINARY RESEARCH, vol. 59, no. 3, pp. 227–231, 2004, [Online]. Available: https://sid.ir/paper/35334/en

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