STUDY OF EFFECTOR ANTIGENS OF IBRVIRUS ON HUMORAL IMMUNITY

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HEMATZADEH F.; KEYVANFAR H.; AHMADI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Paper Information

Journal: Journal of Veterinary Research Year:2007 | Volume:62 | Issue:1 Page(s): 57-61

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Information Journal Paper

Title

STUDY OF EFFECTOR ANTIGENS OF IBRVIRUS ON HUMORAL IMMUNITY

Author(s)

HEMATZADEH F. | KEYVANFAR H. | AHMADI M. | Issue Writer Certificate

Keywords

VIRUSQ3

IBRQ3

SDS-PAGEQ3

IMMUNOBLOTTINGQ4

ANTIGENQ4

Abstract

The objective of this study was to determine the protein pattern and ANTIGENic structure of BHV-1. Ten viral isolates from cows with clinical signs of respiratory infections were selected for this study and confirmed by SN test. All samples were cultured on cell cultures and purified by ultracentrifugation. Purified isolates were electerophorsed using SDS-PAGE method and immunoblotted. Viral ANTIGENs were detected using anti BHV-1 bovine antiserum and molecular weight of the bands were determined. All samples showed identical band number and molecular weight except for one sample which revealed an additional 120-125 KDa band. Also IMMUNOBLOTTING data resulted in identified of twelve bands (150,130,115,97,77,70,55, 45, 40, 38, 32 and 25 KDa) suggesting involvement of these ANTIGENs in evoking humoral immune response in affected cattle.

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References

A SEROLOGICAL SURVEY ON BOVINE RHINOTERACHEITIS VIRUS INFECTION IN CHAHAR MAHAL BAKHTIARY PROVINCE

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APA: Copy

HEMATZADEH, F., KEYVANFAR, H., & AHMADI, M.. (2007). STUDY OF EFFECTOR ANTIGENS OF IBRVIRUS ON HUMORAL IMMUNITY. JOURNAL OF VETERINARY RESEARCH, 62(1), 57-61. SID. https://sid.ir/paper/35371/en

Vancouver: Copy

HEMATZADEH F., KEYVANFAR H., AHMADI M.. STUDY OF EFFECTOR ANTIGENS OF IBRVIRUS ON HUMORAL IMMUNITY. JOURNAL OF VETERINARY RESEARCH[Internet]. 2007;62(1):57-61. Available from: https://sid.ir/paper/35371/en

IEEE: Copy

F. HEMATZADEH, H. KEYVANFAR, and M. AHMADI, “STUDY OF EFFECTOR ANTIGENS OF IBRVIRUS ON HUMORAL IMMUNITY,” JOURNAL OF VETERINARY RESEARCH, vol. 62, no. 1, pp. 57–61, 2007, [Online]. Available: https://sid.ir/paper/35371/en

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