IDENTIFICATION OF SARCOCYSTIS GIGANTEA BY PCR- RFLP

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DALIMI A.; JALOSIAN F.; TAHVILDAR BIDEROUNI F.; GHAFFARIFAR F.

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Journal: Journal of Veterinary Research Year:2010 | Volume:65 | Issue:1 Page(s): 43-46

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Title

IDENTIFICATION OF SARCOCYSTIS GIGANTEA BY PCR- RFLP

Author(s)

DALIMI A. | JALOSIAN F. | TAHVILDAR BIDEROUNI F. | GHAFFARIFAR F. | Issue Writer Certificate

Keywords

SARCOCYSTIS GIGANTEAQ3

SHEEPQ2

PCR-RFLPQ2

IRANQ2

Abstract

Sarcocyst is one of the most important protozoa belonged to apicomplexa. This parasite is prevalent among warm blooded animals throughout the world. In the present work, SARCOCYSTIS GIGANTEA was identified by amplification of 18s rRNA gene using PCR- RFLP. In this regard macroscopic cysts of sarcocystis were collected from esophagus and intra costal muscles of SHEEP in shahriar slaughterhouse. Then genomic DNA of the parasites was extracted from specimens using phenolchloroform method. 18s rRNA gene was amplified with specific primers. For RFLP two restricted enzymes of MSPI and MOBI were used and the patterns were analyzed accordingly. According to the resulted band, all the specimens were identified as SARCOCYSTIS GIGANTEA. This is the first report of molecular identification of sacrocystis gigantea in IRAN.

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APA: Copy

DALIMI, A., JALOSIAN, F., TAHVILDAR BIDEROUNI, F., & GHAFFARIFAR, F.. (2010). IDENTIFICATION OF SARCOCYSTIS GIGANTEA BY PCR- RFLP. JOURNAL OF VETERINARY RESEARCH, 65(1), 43-46. SID. https://sid.ir/paper/35497/en

Vancouver: Copy

DALIMI A., JALOSIAN F., TAHVILDAR BIDEROUNI F., GHAFFARIFAR F.. IDENTIFICATION OF SARCOCYSTIS GIGANTEA BY PCR- RFLP. JOURNAL OF VETERINARY RESEARCH[Internet]. 2010;65(1):43-46. Available from: https://sid.ir/paper/35497/en

IEEE: Copy

A. DALIMI, F. JALOSIAN, F. TAHVILDAR BIDEROUNI, and F. GHAFFARIFAR, “IDENTIFICATION OF SARCOCYSTIS GIGANTEA BY PCR- RFLP,” JOURNAL OF VETERINARY RESEARCH, vol. 65, no. 1, pp. 43–46, 2010, [Online]. Available: https://sid.ir/paper/35497/en

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