Comparison of Neospora caninum Infected Tissues in Aborted Fetal Bovine by PCR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HOSEINI A.; Merat e.; SAMANI S.; Soltan Nezhad s.; Danandeh r.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Veterinary Research Year:2018 | Volume:73 | Issue:3 Page(s): 377-382

Download Full-Text

Persian Verion

View:

900

Download:

Cites:

Information Journal Paper

Title

Comparison of Neospora caninum Infected Tissues in Aborted Fetal Bovine by PCR

Author(s)

Keywords

Neospora caninumQ1

Bovine fetus abortedQ1

PCRQ2

Abstract

BACKGROUND: Neospora caninum is a protozoan intracellular parasite which is considered as one of the main factors for recurrent abortions of dairy cattle in various countries such as Iran. This parasite leads to negative economic impacts such as decline in reproduction, reduced amount of milk, and long calving intervals. OBJECTIVES: There have been numerous tests to determine the cause of abortion. PCR test is considered as a suitable method to specify Neospora caninum DNA and it can determine the DNA in tissue samples and body fluids of the aborted fetus. This study aims to use PCR to evaluate parasites in the tissues of aborted fetuses so as to detect the best tissue for determining the parasite. METHODS: In this study, 82 aborted fetuses in the first six months of 2015 were studied. The tissues were selected from brain, liver, lungs, spleen, kidneys and rennet fluids. The NP21plus primer was used to detect the presence of Neospora caninum in samples. After conducting the PCR Test, samples with 340bp band in Gel electrophoresis were considered as positive. Statistical data from the survey of Neospora caninum’ s presence in selected tissues were evaluated by SAS (version 9. 2) software. RESULTS: Contamination with this parasite was found in 34 brain samples (41. 5%) of aborted fetuses. In 2 (2. 4%) and 4 (4. 9%) of the aborted fetuses, parasite DNA was found in lung and liver tissues along with brain tissues, respectively. CONCLUSIONS: Due to significant difference of infection of brain tissues in comparison to other tissues, our study considers brain tissue as the most appropriate sample for detecting Neospora caninum infection in aborted fetuses in PCR method.

Cites

No record.

References

No record.

Cite

APA: Copy

HOSEINI, A., Merat, e., SAMANI, S., Soltan Nezhad, s., & Danandeh, r.. (2018). Comparison of Neospora caninum Infected Tissues in Aborted Fetal Bovine by PCR. JOURNAL OF VETERINARY RESEARCH, 73(3 ), 377-382. SID. https://sid.ir/paper/35570/en

Vancouver: Copy

HOSEINI A., Merat e., SAMANI S., Soltan Nezhad s., Danandeh r.. Comparison of Neospora caninum Infected Tissues in Aborted Fetal Bovine by PCR. JOURNAL OF VETERINARY RESEARCH[Internet]. 2018;73(3 ):377-382. Available from: https://sid.ir/paper/35570/en

IEEE: Copy

A. HOSEINI, e. Merat, S. SAMANI, s. Soltan Nezhad, and r. Danandeh, “Comparison of Neospora caninum Infected Tissues in Aborted Fetal Bovine by PCR,” JOURNAL OF VETERINARY RESEARCH, vol. 73, no. 3 , pp. 377–382, 2018, [Online]. Available: https://sid.ir/paper/35570/en

Related Journal Papers