ADHESION AND PROLIFERATION OF HORSE FIBROBLAST AND KERATINOCYTE CELLS IN FIBRIN GLUE

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AGHCHEHLOU M.R.; GHAMSARI S.M.; DEHGHAN M.M.; TAHERI MOHAMMAD

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Journal: Journal of Veterinary Research Year:2008 | Volume:62 | Issue:6 Page(s): 391-397

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Title

ADHESION AND PROLIFERATION OF HORSE FIBROBLAST AND KERATINOCYTE CELLS IN FIBRIN GLUE

Author(s)

AGHCHEHLOU M.R. | GHAMSARI S.M. | DEHGHAN M.M. | TAHERI MOHAMMAD | Issue Writer Certificate

Keywords

FIBRIN GLUEQ3

KERATINOCYTEQ3

FIBROBLASTQ3

HORSEQ3

Abstract

Evaluation of autogenous fibrinogen extracted from HORSE blood in FIBRIN GLUE and it's effects on HORSE's FIBROBLAST and KERATINOCYTE cells were preformed. Fibrinogen has been obtained by glycine precipitation technique from HORSE's blood. FIBROBLAST cells were separated by explant culture method from neck skin and KERATINOCYTEs were separated by enzyme digestion from lib samples. Autologous fibrinogen, HORSE`s FIBROBLAST and KERATINOCYTE cells separately mixed with bovine thrombin, calcium gluconate and tranexamic acid. This mixture was uniformly spread on cell culture plate and evaluated for six days. After one day of culture the thickness of FIBRIN GLUE were observed by invert microscope, FIBROBLAST cells in elongated shape were observed in the FIBRIN GLUE in all thickness and KERATINOCYTE cells were flattened and adherent on surface of plate. Both of them proliferated after 3 days. Fibrinogen that was obtained from HORSE blood by glycin precipitation technique can support HORSE FIBROBLASTs as a scaffold and support HORSE KERATINOCYTE proliferation in vitro.

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APA: Copy

AGHCHEHLOU, M.R., GHAMSARI, S.M., DEHGHAN, M.M., & TAHERI, MOHAMMAD. (2008). ADHESION AND PROLIFERATION OF HORSE FIBROBLAST AND KERATINOCYTE CELLS IN FIBRIN GLUE. JOURNAL OF VETERINARY RESEARCH, 62(6), 391-397. SID. https://sid.ir/paper/35657/en

Vancouver: Copy

AGHCHEHLOU M.R., GHAMSARI S.M., DEHGHAN M.M., TAHERI MOHAMMAD. ADHESION AND PROLIFERATION OF HORSE FIBROBLAST AND KERATINOCYTE CELLS IN FIBRIN GLUE. JOURNAL OF VETERINARY RESEARCH[Internet]. 2008;62(6):391-397. Available from: https://sid.ir/paper/35657/en

IEEE: Copy

M.R. AGHCHEHLOU, S.M. GHAMSARI, M.M. DEHGHAN, and MOHAMMAD TAHERI, “ADHESION AND PROLIFERATION OF HORSE FIBROBLAST AND KERATINOCYTE CELLS IN FIBRIN GLUE,” JOURNAL OF VETERINARY RESEARCH, vol. 62, no. 6, pp. 391–397, 2008, [Online]. Available: https://sid.ir/paper/35657/en

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