CHARACTERIZATION OF RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR (G-CSF) PROTEIN BY EXTINCTION COEFFICIENT CALCULATION

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BAGHERIEH FARIBA

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Journal: JORJANI BIOMEDICINE JOURNAL Year:2016 | Volume:3 | Issue:2 Page(s): 0-0

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Title

CHARACTERIZATION OF RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR (G-CSF) PROTEIN BY EXTINCTION COEFFICIENT CALCULATION

Author(s)

BAGHERIEH FARIBA | Issue Writer Certificate

Keywords

EXTINCTION COEFFICIENT (ε)Q2

UV-VIS SPECTROSCOPYQ2

RECOMBINANT GRANULOCYTE-COLONY STIMULATING FACTOR (G-CSF)Q2

SODIUM DODECYL SULFATE POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)Q2

Abstract

Background and Objective: Extinction coefficient is the unique value to each protein and can evaluate the type and purity of recombinant protein produced in laboratories and factories producing recombinant proteins. In this study, a simple method for calculating the extinction coefficient of recombinant protein Granulocyte-Colony Stimulating Factor (G-CSF) is presented.Methods: G-CSF with concentration of 0.5 mg/ml was prepared from Arya Tina Gene. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method was used to check the purity of the protein, then serial dilutions of 1.2 to 1.64 of the protein were prepared and using UV-VIS SPECTROSCOPY the optical density was measured.Results: Electrophoresis result as a single band of 18 kDa was observed. The results of UV-VIS SPECTROSCOPY of serial dilution were plotted as a distinct spectrograms and these were used to plot linear regression curve of the standard concentration of G-CSF.Conclusion: The results of this study shows the extinction coefficient equal to 0.81 M-1 cm-1 for recombinant G-CSF that was accordant with the number reports by international producers of G-CSF and suggests the correct method is used to calculate the physicochemical parameter.

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APA: Copy

BAGHERIEH, FARIBA. (2016). CHARACTERIZATION OF RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR (G-CSF) PROTEIN BY EXTINCTION COEFFICIENT CALCULATION. JORJANI BIOMEDICINE JOURNAL, 3(2), 0-0. SID. https://sid.ir/paper/357724/en

Vancouver: Copy

BAGHERIEH FARIBA. CHARACTERIZATION OF RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR (G-CSF) PROTEIN BY EXTINCTION COEFFICIENT CALCULATION. JORJANI BIOMEDICINE JOURNAL[Internet]. 2016;3(2):0-0. Available from: https://sid.ir/paper/357724/en

IEEE: Copy

FARIBA BAGHERIEH, “CHARACTERIZATION OF RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR (G-CSF) PROTEIN BY EXTINCTION COEFFICIENT CALCULATION,” JORJANI BIOMEDICINE JOURNAL, vol. 3, no. 2, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/357724/en

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