CLONING, EXPRESSION AND FUNCTIONAL ASSESSMENT OF ELASTIN LIKE PROTEIN: A NEW PROTEIN IN MEDICAL ENGINEERING

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Rezaeinasab Neda; ZEINODDINI MEHDI; SAEEDINIA ALI REZA

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Information Journal Paper

Title

CLONING, EXPRESSION AND FUNCTIONAL ASSESSMENT OF ELASTIN LIKE PROTEIN: A NEW PROTEIN IN MEDICAL ENGINEERING

Author(s)

Rezaeinasab Neda | ZEINODDINI MEHDI | SAEEDINIA ALI REZA | Issue Writer Certificate

Keywords

CLONINGQ2

ELASTIN LIKED PROTEINQ1

INVERSE TRANSITION CYCLINGQ1

PURIFICATIONQ3

RECURSIVE DIRECTIONAL LIGATIONQ1

Abstract

Introduction: Elastin like protein or ELP is a synthetic biopolymer consisting the pentapeptide repeats of VPGXG (X can be any amino acid except Pro). This protein is the thermal responsive polypeptide that undergoes a reversible phase transition. At a temperature below the transition temperature (Tt), ELP molecules assume an extended conformation and thus are soluble in aqueous solution; but upon the temperature shift higher than the Tt, however, ELPs become insoluble and form the segregated phase prone to aggrigate’ form. The aim of thisstudy was CLONING, expression and activity of ELP60 according to Tt.Methods: Firstly, ELP10 (150 bp) was synthesized and cloned into pBluescript. Then, Elp60 (900 bp) was produced using RECURSIVE DIRECTIONAL LIGATION (RDL). Finally, ELP60 was subcloned into modified pET25 and ELP60 expression was confirmed using SDS-PAGE method. Also, ELP60 were purified according to Tt and performance of inverse transition cycle (ITC), which confirmed its activity.Results: The results were shown that the pET-ELP60 constructed and ELP60 expressed; it was successfully purified (about 90%) in one step and non-chromatographic method.Conclusion: From produced recombinant protein can be used for simple and easy PURIFICATION of the recombinant protein as pharmaceutical drug, smart drug delivery and tissue engineering.

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APA: Copy

Rezaeinasab, Neda, ZEINODDINI, MEHDI, & SAEEDINIA, ALI REZA. (2017). CLONING, EXPRESSION AND FUNCTIONAL ASSESSMENT OF ELASTIN LIKE PROTEIN: A NEW PROTEIN IN MEDICAL ENGINEERING. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 25(5 ), 361-370. SID. https://sid.ir/paper/35882/en

Vancouver: Copy

Rezaeinasab Neda, ZEINODDINI MEHDI, SAEEDINIA ALI REZA. CLONING, EXPRESSION AND FUNCTIONAL ASSESSMENT OF ELASTIN LIKE PROTEIN: A NEW PROTEIN IN MEDICAL ENGINEERING. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;25(5 ):361-370. Available from: https://sid.ir/paper/35882/en

IEEE: Copy

Neda Rezaeinasab, MEHDI ZEINODDINI, and ALI REZA SAEEDINIA, “CLONING, EXPRESSION AND FUNCTIONAL ASSESSMENT OF ELASTIN LIKE PROTEIN: A NEW PROTEIN IN MEDICAL ENGINEERING,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 25, no. 5 , pp. 361–370, 2017, [Online]. Available: https://sid.ir/paper/35882/en

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