Response Surface Methodology to Optimize Hydrolysis Parameters in Production of Antioxidant Peptides from Wheat Germ Protein by Alcalase Digestion and Identification of Antioxidant Peptides by LC-MS/MS

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KARAMI Z.; PEIGHAMBARDOUST S.H.; HESARI J.; AKBARI ADERGANI B.

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Paper Information

Journal: JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST) Year:2019 | Volume:21 | Issue:4 Page(s): 829-844

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Title

Response Surface Methodology to Optimize Hydrolysis Parameters in Production of Antioxidant Peptides from Wheat Germ Protein by Alcalase Digestion and Identification of Antioxidant Peptides by LC-MS/MS

Author(s)

KARAMI Z. | PEIGHAMBARDOUST S.H. | HESARI J. | AKBARI ADERGANI B. | Issue Writer Certificate

Keywords

ABTS assayQ3

Amino acids profilesQ3

Bioactive peptidesQ3

RSMQ3

Abstract

The antioxidant capacity of wheat germ protein hydrolyzed by Alcalase was optimized using Response Surface Methodology (RSM). The optimum hydrolyzing parameters were found at temperature of 52. 28° C, time 233 minutes, and E/S 1. 46 %. The Amino acids profiles of intact and hydrolyzed proteins showed that Wheat Germ Protein Hydrolysate (WGPH) had higher percentage of hydrophobic amino acids than that of intact protein. WGPH prepared in optimum condition was fractionated by RP-HPLC. The obtained fractions were subjected to ABTS assay for antioxidant capacity evaluation. The fraction with higher antioxidant value was then exposed to further analysis by LC-ESI/MS/MS. The sequences of the peptides were found to be TVGGAPAGRIVME (1257. 66 Da) and GNPIPREPGQVPAY (1494. 77 Da).

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APA: Copy

KARAMI, Z., PEIGHAMBARDOUST, S.H., HESARI, J., & AKBARI ADERGANI, B.. (2019). Response Surface Methodology to Optimize Hydrolysis Parameters in Production of Antioxidant Peptides from Wheat Germ Protein by Alcalase Digestion and Identification of Antioxidant Peptides by LC-MS/MS. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), 21(4 ), 829-844. SID. https://sid.ir/paper/359373/en

Vancouver: Copy

KARAMI Z., PEIGHAMBARDOUST S.H., HESARI J., AKBARI ADERGANI B.. Response Surface Methodology to Optimize Hydrolysis Parameters in Production of Antioxidant Peptides from Wheat Germ Protein by Alcalase Digestion and Identification of Antioxidant Peptides by LC-MS/MS. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST)[Internet]. 2019;21(4 ):829-844. Available from: https://sid.ir/paper/359373/en

IEEE: Copy

Z. KARAMI, S.H. PEIGHAMBARDOUST, J. HESARI, and B. AKBARI ADERGANI, “Response Surface Methodology to Optimize Hydrolysis Parameters in Production of Antioxidant Peptides from Wheat Germ Protein by Alcalase Digestion and Identification of Antioxidant Peptides by LC-MS/MS,” JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), vol. 21, no. 4 , pp. 829–844, 2019, [Online]. Available: https://sid.ir/paper/359373/en

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