GENOTYPING AND ANTIBIOTIC RESISTANCE OF ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM PATIENTS HOSPITALIZED IN TEACHING HOSPITALS OF SHAHREKORD BY PULSED- FIELD GEL ELECTROPHORSIS

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GHOLIPUR ABOLFAZL; ANSARI NAJMEH; DAMAVANDI MOHAMMADSADEGH; RABIEI MOHAMMAD; MIRNEZHAD REZA

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2017 | Volume:25 | Issue:3 Page(s): 241-251

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Title

GENOTYPING AND ANTIBIOTIC RESISTANCE OF ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM PATIENTS HOSPITALIZED IN TEACHING HOSPITALS OF SHAHREKORD BY PULSED- FIELD GEL ELECTROPHORSIS

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Keywords

GENOTYPINGQ2

ACINETOBACTER BAUMANNIIQ1

PULSED- FIELD GEL ELECTROPHORSISQ1

Abstract

Introduction: Acinetobacters especially ACINETOBACTER BAUMANNII causing nosocomial infections in hospitals intensive care units and can cause a variety of hospital infections such as bacteremia, meningitis, pneumonia and urinary tract infections. There are several molecular techniques for microbial GENOTYPING, among them Pulsed- Field Gel Electrophoreses is introduced as the gold standard for sub typing of bacteria. The aim of this study was investigating the molecular typing of A. baumannii strains with PFGE as well as the relationship between common types available and their antibiotic resistance.Methods: In this descriptive - analysis study, 50 ACINETOBACTER BAUMANNII were confirmed with cultivation methods and biochemical tests. Then, bacteria were detected using PFGE typing and the results were compared with the results of antibiotic resistance.Results: The results showed that all isolates had multiple resistance. The highest sensitivity was observed for tobramycin (52%), gentamicin (36%) and moropenem (32%).The results of this study showed that A. baumannii strains isolated from Shahrekord hospitals were in seven different genetic patterns that two of these patterns were sporadic and the genetic patterns were different in each hospital.Conclusion: Although variations among strains of ACINETOBACTER BAUMANNII were observed by using PFGE in Shahrekord, but no epidemic strain was detected among them. In terms of resistance to commonly used antibiotics were also different patterns.

Cites

Frequency of Resistance Phenotypes and Genotype of Acinetobacter Baumannii and Molecular Typing by PFGE in Patients Admitted to the Intensive Care Units of Zanjan Hospitals

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APA: Copy

GHOLIPUR, ABOLFAZL, ANSARI, NAJMEH, DAMAVANDI, MOHAMMADSADEGH, RABIEI, MOHAMMAD, & MIRNEZHAD, REZA. (2017). GENOTYPING AND ANTIBIOTIC RESISTANCE OF ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM PATIENTS HOSPITALIZED IN TEACHING HOSPITALS OF SHAHREKORD BY PULSED- FIELD GEL ELECTROPHORSIS. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 25(3), 241-251. SID. https://sid.ir/paper/36039/en

Vancouver: Copy

GHOLIPUR ABOLFAZL, ANSARI NAJMEH, DAMAVANDI MOHAMMADSADEGH, RABIEI MOHAMMAD, MIRNEZHAD REZA. GENOTYPING AND ANTIBIOTIC RESISTANCE OF ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM PATIENTS HOSPITALIZED IN TEACHING HOSPITALS OF SHAHREKORD BY PULSED- FIELD GEL ELECTROPHORSIS. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;25(3):241-251. Available from: https://sid.ir/paper/36039/en

IEEE: Copy

ABOLFAZL GHOLIPUR, NAJMEH ANSARI, MOHAMMADSADEGH DAMAVANDI, MOHAMMAD RABIEI, and REZA MIRNEZHAD, “GENOTYPING AND ANTIBIOTIC RESISTANCE OF ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM PATIENTS HOSPITALIZED IN TEACHING HOSPITALS OF SHAHREKORD BY PULSED- FIELD GEL ELECTROPHORSIS,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 25, no. 3, pp. 241–251, 2017, [Online]. Available: https://sid.ir/paper/36039/en

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