Evaluation of microRNAs; mir223, mir222 and mir92a levels in the Platelet-derived microparticles in the Platelet concentrates produced by Platelet Rich Plasma method during storage

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Abbaszadeh G.; ATARODI K.; MOUSAVI HOSSEINI K.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: The Scientific Journal of Iranian Blood Transfusion Year:2020 | Volume:17 | Issue:2 Page(s): 100-112

Download Full-Text

Persian Verion

View:

431

Download:

Cites:

Information Journal Paper

Title

Evaluation of microRNAs; mir223, mir222 and mir92a levels in the Platelet-derived microparticles in the Platelet concentrates produced by Platelet Rich Plasma method during storage

Author(s)

Abbaszadeh G. | ATARODI K. | MOUSAVI HOSSEINI K. | Issue Writer Certificate

Keywords

MicroRNAQ1

PlateletsQ3

PlasmaQ3

Abstract

Background and Objectives: Platelets release microparticles containing cellular components, including MicroRNAs, during storage. Assessment of these MicroRNAs is one of the markers for evaluation of platelet storage lesions. The aim of the present study was to evaluate the level of changes in the expression of mir-223, mir-92a and mir-222 during storage in Platelets prepared by platelet rich Plasma method. Materials and Methods: In this experimental study, 5 platelet concentrates prepared by PRP method were collected from blood donors and stored at 22° C for 5 days with agitation. Changes in the expression levels of mir-223, mir-92a and mir-222 were determined using qRT-PCR method on days zero, three and five of storage. The results were analyzed using paired-sample-T test using GenEX version 7 software. Results: It was found that the expression of miR-223 gradually increased during the third and fifth days compared to the day 0 of platelet concentrate storage (p < 0. 05). The expression of mir-92a also significantly increased on the third and the fifth days compared to day 0 of storage (p < 0. 05). However, the expression of mir-222 gradually decreased over the fifth day of storage (p < 0. 05). Conclusions: This study showed that the determination of miRNAs in the platelet-derived microparticles with common markers such as platelet count, MPV determination, platelet volume determination, leukocyte count, swirling assay, and pH measurement can be useful tools for identifying cellular damage associated with platelet storage lesion and maybe potential indicators for evaluating the quality and viability of Platelets stored in vitro conditions.

Cites

No record.

References

Cite

APA: Copy

Abbaszadeh, G., ATARODI, K., & MOUSAVI HOSSEINI, K.. (2020). Evaluation of microRNAs; mir223, mir222 and mir92a levels in the Platelet-derived microparticles in the Platelet concentrates produced by Platelet Rich Plasma method during storage. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 17(2 ), 100-112. SID. https://sid.ir/paper/360938/en

Vancouver: Copy

Abbaszadeh G., ATARODI K., MOUSAVI HOSSEINI K.. Evaluation of microRNAs; mir223, mir222 and mir92a levels in the Platelet-derived microparticles in the Platelet concentrates produced by Platelet Rich Plasma method during storage. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2020;17(2 ):100-112. Available from: https://sid.ir/paper/360938/en

IEEE: Copy

G. Abbaszadeh, K. ATARODI, and K. MOUSAVI HOSSEINI, “Evaluation of microRNAs; mir223, mir222 and mir92a levels in the Platelet-derived microparticles in the Platelet concentrates produced by Platelet Rich Plasma method during storage,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 17, no. 2 , pp. 100–112, 2020, [Online]. Available: https://sid.ir/paper/360938/en

Related Journal Papers