Genetic diversity assessment of Agropyron cristatum accessions using ISSR marker

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Zarafshani F.; SAFARI H.; ETMINAN A.R.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2020 | Volume:15 | Issue:1 Page(s): 69-73

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Title

Genetic diversity assessment of Agropyron cristatum accessions using ISSR marker

Author(s)

Zarafshani F. | SAFARI H. | ETMINAN A.R. | Issue Writer Certificate

Keywords

Molecular markerQ1

AgropyronQ1

Genetic diversityQ1

Geographic distributionQ1

Abstract

Genetic variation was investigated for the number of 15 Agropyron cristatum accessions using the number of 15 ISSR primers. The number of 12 primers can be scored. The ISSR primers produced the number of 71 bands. The polymorphism has been observed for the number of 66 bands. The primers of IS1 and UBC857 presented the highest number of bands, i. e. eight bands; while the IS7 and UBC869 showed the lowest number which is only three bands. The primer of UBC844 were showed the lowest polymorphism percentage (83. 33%) and IS2, IS6, IS7, UBC848, UBC865, UBC868 and UBC869 had the highest polymorphism percentage (100%). The polymorphism percentage average was 94. 15%. The average of bands was 5. 92 in each primer for 15 genotypes and the average of polymorphic bands was 5. 50. IS6, UBC865 and UBC869 can determinate genetic distance of genotypes better than other primers. The genotypes of G4 with G2 had the most similarity and G1 with G8 had the lowest similarity. The results of UPGMA cluster analysis based on Jacard's similarity coefficient were fallen the genotypes in three groups, which confirmed by principal coordinate analysis and molecular variance analysis. The Genetic diversity of the populations was matched based on the studied marker with their Geographic distribution, and the populations of Gorgan and Isfahan were similar, which, on the other hand, showed greater distance with Karaj populations.

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APA: Copy

Zarafshani, F., SAFARI, H., & ETMINAN, A.R.. (2020). Genetic diversity assessment of Agropyron cristatum accessions using ISSR marker. MODERN GENETICS JOURNAL (MGJ), 15(1 ), 69-73. SID. https://sid.ir/paper/362091/en

Vancouver: Copy

Zarafshani F., SAFARI H., ETMINAN A.R.. Genetic diversity assessment of Agropyron cristatum accessions using ISSR marker. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2020;15(1 ):69-73. Available from: https://sid.ir/paper/362091/en

IEEE: Copy

F. Zarafshani, H. SAFARI, and A.R. ETMINAN, “Genetic diversity assessment of Agropyron cristatum accessions using ISSR marker,” MODERN GENETICS JOURNAL (MGJ), vol. 15, no. 1 , pp. 69–73, 2020, [Online]. Available: https://sid.ir/paper/362091/en

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