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Information Journal Paper

Title

Genetic variability of Malva neglecta ecotype using ISSR molecular markers

Pages

  00-00

Abstract

 In this research, the genetic diversity of 18 Malva (Malva neglecta) from the gene bank of forests and rangelands was evaluated. DNA extraction was performed using CTAB method, and genetic variation was investigated with 15 ISSR markers. All of the ISSR primers showed 99 visible bands in which four bands were similar patterns. The IS5 and IS6 primers had the most number of bands with 12 and 11 bands respectively, while UBC807 and UBC867 with two bands showed the least band numbers. The Polymorphic information content (PIC), marker index (MI), EMR and RP indices were calculated for all primers. With this point of view, IS5 and IS6 were the best primers to identify variability among these Malva. Total genetic similarity based on these primers was 69 percent. The greatest genetic similarity was between G4 with G9 ecotype. The lowest genetic distance was between G1 and G9 ecotype. Cluster analysis based Jakard coefficient by UPGMA was classified all genotype to tree groups and this clustering was confirmed by principal coordinate (PCo) and analysis of molecular variance (AMOVA). Portion of between group variance was just 29 percent of total variance.

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    APA: Copy

    NOORIAN, A.M., & SHIRVANI, H.. (2020). Genetic variability of Malva neglecta ecotype using ISSR molecular markers. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 32(4 ), 00-00. SID. https://sid.ir/paper/363147/en

    Vancouver: Copy

    NOORIAN A.M., SHIRVANI H.. Genetic variability of Malva neglecta ecotype using ISSR molecular markers. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2020;32(4 ):00-00. Available from: https://sid.ir/paper/363147/en

    IEEE: Copy

    A.M. NOORIAN, and H. SHIRVANI, “Genetic variability of Malva neglecta ecotype using ISSR molecular markers,” JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 32, no. 4 , pp. 00–00, 2020, [Online]. Available: https://sid.ir/paper/363147/en

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