CLONING AND EXPRESSION OF FUSION GENES OF DOMAIN A-1 PROTECTIVE ANTIGEN OF BACILLUS ANTHRACIS AND SHIGELLA ENTEROTOXIN B SUBUNIT (STXB) IN E. COIL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

AHMADI A.H.; HONARI H.; MINAEI M.E.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2015 | Volume:22 | Issue:6 Page(s): 1702-1711

Download Full-Text

Persian Verion

View:

880

Download:

Cites:

Information Journal Paper

Title

CLONING AND EXPRESSION OF FUSION GENES OF DOMAIN A-1 PROTECTIVE ANTIGEN OF BACILLUS ANTHRACIS AND SHIGELLA ENTEROTOXIN B SUBUNIT (STXB) IN E. COIL

Author(s)

AHMADI A.H. | HONARI H. | MINAEI M.E. | Issue Writer Certificate

Keywords

BACILLUS ANTHRACISQ2

PROTECTIVE ANTIGEN (PA20)Q2

SHIGELLA DYSENTERIAE TYPE 1Q1

SHIGELLA ENTEROTOXIN B SUBUNIT (STXB)Q2

Abstract

Introduction: Shigella enterotoxin (STxB) is one of the major virulent factors in SHIGELLA DYSENTERIAE TYPE 1 and E. coli O157:H7, which its immunogenicity, adjuvant and delivery characteristic have been proven. Anthrax is a common disease in humans and animals and identifies domain a-1 antibodies of protective antigen (PA) which is 62% of the PA antigens of BACILLUS ANTHRACIS. Therefore, the purpose of this study was to investigate the expression of recombinant protein domain a-1 protective antigen (PA20) of BACILLUS ANTHRACIS with Shigella enterotoxin B subunit (STxB) in E. coli.Methods: In this experimental study, primers of pa 20 gene were designed for replacement in stxB-ipaD gene cassette. PCR was performed in order to amplify the fragment and the amplified fragments were cloned into pGEM-Teasy vector. NdeI and SalI restriction enzymes cut pa20 genes and finally the genes pa20 were fused with genes stxB. PET28a (+) Vector containing the gene cassette pa20-stxB was transformed into E. coli strain BL21 (DE3) and expression of gene cassette was studied.Results: pa20-stxB fused genes were confirmed in the expression vector pET28a (+) by PCR, enzyme digestion and Sequencing. The produced recombinant proteins were confirmed by SDS-PAGE and Western blotting.Conclusion: The findings of the current study revealed that this antigen can be raised as an anti-cancer and recombinant vaccine candidate against types of Shigella, Escherichia coli and BACILLUS ANTHRACIS which can be due to such factors as identification of antigen(PA) by antibody PA20, its apoptosis induction properties, property of immunogenicity, adjuvant and delivery of STxB protein and high expression levels of Gb3 in human cancer cells.

Cites

No record.

References

No record.

Cite

APA: Copy

AHMADI, A.H., HONARI, H., & MINAEI, M.E.. (2015). CLONING AND EXPRESSION OF FUSION GENES OF DOMAIN A-1 PROTECTIVE ANTIGEN OF BACILLUS ANTHRACIS AND SHIGELLA ENTEROTOXIN B SUBUNIT (STXB) IN E. COIL. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 22(6), 1702-1711. SID. https://sid.ir/paper/36320/en

Vancouver: Copy

AHMADI A.H., HONARI H., MINAEI M.E.. CLONING AND EXPRESSION OF FUSION GENES OF DOMAIN A-1 PROTECTIVE ANTIGEN OF BACILLUS ANTHRACIS AND SHIGELLA ENTEROTOXIN B SUBUNIT (STXB) IN E. COIL. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2015;22(6):1702-1711. Available from: https://sid.ir/paper/36320/en

IEEE: Copy

A.H. AHMADI, H. HONARI, and M.E. MINAEI, “CLONING AND EXPRESSION OF FUSION GENES OF DOMAIN A-1 PROTECTIVE ANTIGEN OF BACILLUS ANTHRACIS AND SHIGELLA ENTEROTOXIN B SUBUNIT (STXB) IN E. COIL,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 22, no. 6, pp. 1702–1711, 2015, [Online]. Available: https://sid.ir/paper/36320/en

Related Journal Papers