Comparing the Effect of Homocysteinylated and Normal Forms of Substance P on the Proliferation of PC12 Cell-line in the Presence of Cysteine

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Davoud Manesh S.; Mohammadian M.J.

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Journal: JOURNAL OF CELL & TISSUE Year:2018 | Volume:9 | Issue:3 Page(s): 250-260

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Title

Comparing the Effect of Homocysteinylated and Normal Forms of Substance P on the Proliferation of PC12 Cell-line in the Presence of Cysteine

Author(s)

Davoud Manesh S. | Mohammadian M.J. | Issue Writer Certificate

Keywords

Cancer cellsQ2

HomocysteineQ1

PC12 cell lineQ1

Substance PQ1

Abstract

Aim: In the present study, the effects of homocysteinylated and normal forms of Substance P were investigated on the proliferation of PC12 cell-line in the presence of cysteine. Material and methods: Cells were cultured in an RPMI-Glutamax medium containing 10% FBS and metabolic activity was assessed in the presence of different concentrations of peptide and cysteine using the MTT assay. Afterward, the morphological changes were determined using phase-contrast microscopy. In order to evaluate the protective effect of homocysteinylated peptide against H2O2, cells were treated with 150 μ g/ml H2O2 for 3 h, and their nitric oxide production was assessed using the Griess colorimetric assay. Results: findings showed that two forms of Substance P, at selected concentrations, caused a significant increase in proliferation of PC12 cells and a decrease in the amount of NO production in response to H2O2 toxicity. At a concentration of 6-10 M, homocysteinylated and normal forms of Substance P increased the rate of PC12 proliferation up to 78. 65% and 38. 55 % and decreased the levels of NO up to 36% and 14%, respectively. This indicates that homocysteinylated form of Substance P is more potent (~40%) than the normal form. The percentage of protection for homocysteinylated Substance P was about 22% higher than the normal form. Conclusion: It seems that homocysteinylated Substance P can increase the growth rate of Cancer cells and promote the disease course through the reduction in NO production in pathological conditions.

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APA: Copy

Davoud Manesh, S., & Mohammadian, M.J.. (2018). Comparing the Effect of Homocysteinylated and Normal Forms of Substance P on the Proliferation of PC12 Cell-line in the Presence of Cysteine. JOURNAL OF CELL & TISSUE, 9(3 ), 250-260. SID. https://sid.ir/paper/363850/en

Vancouver: Copy

Davoud Manesh S., Mohammadian M.J.. Comparing the Effect of Homocysteinylated and Normal Forms of Substance P on the Proliferation of PC12 Cell-line in the Presence of Cysteine. JOURNAL OF CELL & TISSUE[Internet]. 2018;9(3 ):250-260. Available from: https://sid.ir/paper/363850/en

IEEE: Copy

S. Davoud Manesh, and M.J. Mohammadian, “Comparing the Effect of Homocysteinylated and Normal Forms of Substance P on the Proliferation of PC12 Cell-line in the Presence of Cysteine,” JOURNAL OF CELL & TISSUE, vol. 9, no. 3 , pp. 250–260, 2018, [Online]. Available: https://sid.ir/paper/363850/en

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