DETECTION OF METALLO-BETA-LACTAMASE BLAVIM-1 GENE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA IN MASHHAD

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NAKHAEI MOGHADDAM MAHBOOBEH; HOSSEINI HASANABADY MARYAM

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2016 | Volume:24 | Issue:1 Page(s): 74-82

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Title

DETECTION OF METALLO-BETA-LACTAMASE BLAVIM-1 GENE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA IN MASHHAD

Author(s)

NAKHAEI MOGHADDAM MAHBOOBEH | HOSSEINI HASANABADY MARYAM | Issue Writer Certificate

Keywords

PSEUDOMONAS AERUGINOSAQ2

METALLO-BETA-LACTAMASEQ2

BLAVIM-1 GENEQ1

ANTIBIOTIC RESISTANCEQ2

Abstract

Introduction: Carbapenems are one of the latest drugs for treatment of threatening infections of PSEUDOMONAS AERUGINOSA. Resistance to these antibiotics is sometimes caused by carbapnmase class B, such as VIM metallobeta-lactamase (MBL) that is growing in the communities. The aim of this study was to detect the of metallobeta-lactamaseblaVIM-1 gene among clinical isolates of PSEUDOMONAS AERUGINOSA in Mashhad.Methods: In this cross-sectional study, 70 isolates of PSEUDOMONAS AERUGINOSA were collected from samples of inpatients at hospitals in Mashhad. Bacteria were identified by conventional biochemical methods. Antibiotic susceptibility testing was examined in accordance with the Kirby-Bauer method. METALLO-BETA-LACTAMASE producing isolates were identified using a combination disc of imipenem and EDTA. Then, polymerase chain reaction was performed using specific primers to detectblaVIM-1 gene.Results: 25 (35.7%) out of 70 isolated bacteria had metallo-beta- lactamase, which BLAVIM-1 GENE were detected in the genome of 8 isolates.65.6% of resistant bacteria to imipenem produced METALLO-BETA-LACTAMASE (MBL). A higher percentage of MBL- producing isolates were resistant to imipenem, meropenem, carbenicillin and cefotaxime (p<0.05).Conclusion: The results of the study showed that ANTIBIOTIC RESISTANCE among examined clinical isolates of Paeruginosa was high and VIM-type METALLO-BETA-LACTAMASE was detected among them. Identification of bacteria with METALLO-BETA-LACTAMASE (MBL) is very important in the prevention and treatment of infections resistant to antibiotics.

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APA: Copy

NAKHAEI MOGHADDAM, MAHBOOBEH, & HOSSEINI HASANABADY, MARYAM. (2016). DETECTION OF METALLO-BETA-LACTAMASE BLAVIM-1 GENE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA IN MASHHAD. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 24(1), 74-82. SID. https://sid.ir/paper/36461/en

Vancouver: Copy

NAKHAEI MOGHADDAM MAHBOOBEH, HOSSEINI HASANABADY MARYAM. DETECTION OF METALLO-BETA-LACTAMASE BLAVIM-1 GENE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA IN MASHHAD. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;24(1):74-82. Available from: https://sid.ir/paper/36461/en

IEEE: Copy

MAHBOOBEH NAKHAEI MOGHADDAM, and MARYAM HOSSEINI HASANABADY, “DETECTION OF METALLO-BETA-LACTAMASE BLAVIM-1 GENE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA IN MASHHAD,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 24, no. 1, pp. 74–82, 2016, [Online]. Available: https://sid.ir/paper/36461/en

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