CISPLATIN CYTOTOXICITY ON CHINESS HAMSTER OVARIAN CARCINOMA (CHO) CELL LINE

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HOSSEINI SHIRAZI S.F.; EFTEKHARI MEHRDAD

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Journal: Researcher Bulletin of Medical Sciences Year:2004 | Volume:9 | Issue:4 (40) Page(s): 191-198

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Title

CISPLATIN CYTOTOXICITY ON CHINESS HAMSTER OVARIAN CARCINOMA (CHO) CELL LINE

Author(s)

HOSSEINI SHIRAZI S.F. | EFTEKHARI MEHRDAD | Issue Writer Certificate

Keywords

CHINESE HAMESTER OVARIAN CARCINOMA CELL LINE (CHO) CISPLATIN CYTOTOXICITY CLONOGENIC ASSAYQ4

Abstract

Background: Cisplatin is one of the most widely used drugs in cancer chemotherapy. This drug has shown a great therapeutic advantage on the treatment of advanced ovarian carcinoma, bladder, head and neck, lung and some childhood neoplasms. Cell culture is one of the most suitable methods for the assesment of chemical’s effects on cell proliferation and growth, specially the cancer ones. This study was performed to investigate the effects of cisplatin on cancer cells growth. Materials and methods: Chinese hamster ovarian carcinoma cell line (CHO) has been grown in DMEM/F12 media supplied with 10% FCS and in the humified 5% CO2, 37°C incubator. Growth curve of these cells has been drawn in control and cells exposed to the highest dose-response followed concentration for one hour. Cisplatin cytotoxicity has been calculated using clonogenic assay. Prism© V.4 software has been used for statistical calculation and to draw related graphs on computer.Results: CHO growth curve followed the general pattern of logarithmic and plateaue phases. Doubling time of control cells was calculated equal to be 18.26±2.6 hour. Cisplatin cytotoxicity on this cell line followed the dose-response pattern up to 1 mg/ml. Cisplatin exposed concentration didn’t increase CHO cytotoxicity after then. Cisplatin exposure to CHO cells decreased the plating efficacy of these cells for 37% (p<0.05). Conclusion: Cisplatin affects CHO growth parameters at all different phases of plating efficacy, logarithmic and plateau. The most critical effect is however on the plating efficacy, which is consequently affecting other phases. This is what has been shown for the first time in this study. Such an observation might suggest cisplatin usage as an anti-implating agent for cancer cells, which may result in the inhibition of secondary tumour progression of patients. Other novel discovery of this study shows that although cisplatin cytotoxicity is following a direct dose-response pattern within the clinical concentrations of this drug, but has a limited range for such a direct relationship. Cisplatin does not have a significant cytotoxic effect in doses higher than 1 mg/ml in CHO cells. A similar comparative study in other cell lines and more deep investigation on possible mechanisms for these observations is suggested.

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