EXPRESSION OF BLF1-STX B GENE CASSETTE IN E. COLI AND INVESTIGATION ANTIBODY TITER IN MICE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MASOUDI KERAHROUDI MEHDI; HONARI HOSSIEN; ABDOLLAHI MASOUD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2017 | Volume:24 | Issue:11 Page(s): 876-886

Download Full-Text

Persian Verion

View:

604

Download:

Cites:

Information Journal Paper

Title

EXPRESSION OF BLF1-STX B GENE CASSETTE IN E. COLI AND INVESTIGATION ANTIBODY TITER IN MICE

Author(s)

MASOUDI KERAHROUDI MEHDI | HONARI HOSSIEN | ABDOLLAHI MASOUD | Issue Writer Certificate

Keywords

BURKHOLDERIA PSEUDOMALLEIQ1

MELIOIDOSISQ1

SHIGELLAQ2

EIF4AQ1

Abstract

Background and Objectives: One of the ways to strengthen the effect of vaccines is using the adjuvant. STxB has a carrier and adjuvant role that we can fuse it with vaccine candidate antigens and produce efficient vaccines. BLF1 has a role in the pathogenesis and infection by BURKHOLDERIA PSEUDOMALLEI, that can be studied when fused with stxB.The aim of this study expression blf1-stxB gene Cassette in E. coli and antibody production in mice.Methods:In this study the synthetic gene in pUC57 plasmid was purchased from Nedaye Fan COR. pUC57 plasmid containing blf1 with BamHI, SalI restriction enzyme sites was subcloned in pET28a(+)-stxB expression vector and transformed into E. coli BL21 DE3. expression blf1-stxB gene Cassette was induced by IPTG. After extraction by affinity chromatography, the recombinant protein was injected four times to mice.Results: In this study, blf1-StxB cloned gene in pET28a(+) expression vector was approved by PCR and enzymatic analysis. Also recombinant protein confirmed by SDS-PAGE and Western blotting. Then antibody produced from the mice serum, were isolated and confirmed by ELISA.Conclusion: Given that BLF1 protein has the ability to stop protein synthesis and STxB has a carrier and adjuvant role, also with respect to antibody titers produced, it’s as a vaccine candidate against B. pseudomallei and SHIGELLA dysenteriae.

Cites

Comparison of the Titers of Produced Antibodies against BLF1 and BLF1-STxB Recombinant Proteins in Laboratory Rats

References

No record.

Cite

APA: Copy

MASOUDI KERAHROUDI, MEHDI, HONARI, HOSSIEN, & ABDOLLAHI, MASOUD. (2017). EXPRESSION OF BLF1-STX B GENE CASSETTE IN E. COLI AND INVESTIGATION ANTIBODY TITER IN MICE. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 24(11), 876-886. SID. https://sid.ir/paper/36579/en

Vancouver: Copy

MASOUDI KERAHROUDI MEHDI, HONARI HOSSIEN, ABDOLLAHI MASOUD. EXPRESSION OF BLF1-STX B GENE CASSETTE IN E. COLI AND INVESTIGATION ANTIBODY TITER IN MICE. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;24(11):876-886. Available from: https://sid.ir/paper/36579/en

IEEE: Copy

MEHDI MASOUDI KERAHROUDI, HOSSIEN HONARI, and MASOUD ABDOLLAHI, “EXPRESSION OF BLF1-STX B GENE CASSETTE IN E. COLI AND INVESTIGATION ANTIBODY TITER IN MICE,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 24, no. 11, pp. 876–886, 2017, [Online]. Available: https://sid.ir/paper/36579/en

Related Journal Papers