The Frequency of Sidrophores irp2 and iroN encoding Genes in Escherichia Coli Clinical Isolates

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Faraz Firozeh; MIRZAEI MOHSEN

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Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2019 | Volume:37 | Issue:560 Page(s): 1448-1453

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Title

The Frequency of Sidrophores irp2 and iroN encoding Genes in Escherichia Coli Clinical Isolates

Author(s)

Faraz Firozeh | MIRZAEI MOHSEN | Issue Writer Certificate

Keywords

Escherichia coliQ1

Urinary tract infectionQ2

SiderophoresQ1

VaccineQ2

IranQ2

Abstract

Background: The most important genes that exacerbate Urinary tract infection are the siderophore genes, iroN and irp2. The aim of this study was to investigate the prevalence of siderophore genes irp2 and iroN in order to provide a suitable solution for the management of the bacteria carrying the mentioned gene as well as the preparation of a Vaccine. Methods: This experimental study was done in Boroujerd Branch, Islamic Azad University, Iran. 100 samples were obtained from clinical laboratories in Boroujerd City. After identification of DNA extraction by boiling method, polymerase chain reaction (PCR) was performed using specific primers. Findings: Frequency of siderophore genes in the studied samples showed that 58 (58%) had irp2 gene, 28 (28%) had iroN gene, 4 (4%) had two genes, and 10 (10%) of both genes were negative. Conclusion: The results of our study showed that the prevalence of both Siderophores irp2 and iroN genes was high in the cultured samples, but Siderophores irp2 had higher prevalence. Moreover, given the high prevalence of these genes in women on the one hand, and the high prevalence of broad-spectrum antibiotic-resistant bacteria on the other hand, it is likely that this and other similar studies could be a prelude to Vaccine preparation for bacterial surface antigens of Escherichia coli.

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APA: Copy

Faraz, Firozeh, & MIRZAEI, MOHSEN. (2019). The Frequency of Sidrophores irp2 and iroN encoding Genes in Escherichia Coli Clinical Isolates. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 37(560 ), 1448-1453. SID. https://sid.ir/paper/366132/en

Vancouver: Copy

Faraz Firozeh, MIRZAEI MOHSEN. The Frequency of Sidrophores irp2 and iroN encoding Genes in Escherichia Coli Clinical Isolates. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2019;37(560 ):1448-1453. Available from: https://sid.ir/paper/366132/en

IEEE: Copy

Firozeh Faraz, and MOHSEN MIRZAEI, “The Frequency of Sidrophores irp2 and iroN encoding Genes in Escherichia Coli Clinical Isolates,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 37, no. 560 , pp. 1448–1453, 2019, [Online]. Available: https://sid.ir/paper/366132/en

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