SUBCLONING AND EXPRESSION OF SO6 GENE, SAPONARIA OFFICINALIS PLANT IN E.COLI AND INVESTIGATION OF ANTIBODY TITER IN RATS

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ABDOLLAHI MASOUD; HONARI HOSSIEN; NAZARIAN SHAHRAM; MASOUDI KERAHROUDI MEHDI

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2017 | Volume:24 | Issue:12 Page(s): 1024-1033

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Title

SUBCLONING AND EXPRESSION OF SO6 GENE, SAPONARIA OFFICINALIS PLANT IN E.COLI AND INVESTIGATION OF ANTIBODY TITER IN RATS

Author(s)

ABDOLLAHI MASOUD | HONARI HOSSIEN | NAZARIAN SHAHRAM | MASOUDI KERAHROUDI MEHDI | Issue Writer Certificate

Keywords

N-GLYCOSIDASEQ1

SAPONARIA OFFICINALISQ1

RIPQ1

SO6Q1

Abstract

Introduction: Plant ribosome inactivating proteins act as N-GLYCOSIDASE enzyme and produce by several family of Caryophyllaceae such as SAPONARIA OFFICINALIS. Different Isoforms of RIPs expressed by SAPONARIA OFFICINALIS. SO6 isoform depurinate Adenine 4324 in the conserved GAGA loop of 28SrRNA and disrupts protein synthesis. The aim of this study was expression of SO6 isoform inE.coli and investigation of antibody titer in rats.Methods: In this experimental study, SO6 synthetic gene was excised from recombinant pUC57- SO6 plasmid with Bam HI and Sal I restriction enzymes and subcloned into pET28a (+) expression vector. The expression of recombinant protein was induced by IPTG. Recombinant SO6 was purified by nickel affinity chromatography. Western blotting was performed to confirm the recombinant protein. Rats were immunized intraperitoneal with purified protein and IgG serum titer was assayed by ELISA.Results: PCR reaction and enzyme digestion confirmed subcloning of SO6 gene into pET28a (+) expression vector. A 29.5kDa protein band on SDS-PAGE showed a high level of recombinant protein expression. Polyclonal antibodies recognized SO6. ELISA confirmed significant antibody titer after injection of protein in test group compared with the control group.Conclusion: The recombinant purified SO6 antigen can be used for anti-cancer and vaccine candidate research.

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APA: Copy

ABDOLLAHI, MASOUD, HONARI, HOSSIEN, NAZARIAN, SHAHRAM, & MASOUDI KERAHROUDI, MEHDI. (2017). SUBCLONING AND EXPRESSION OF SO6 GENE, SAPONARIA OFFICINALIS PLANT IN E.COLI AND INVESTIGATION OF ANTIBODY TITER IN RATS. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 24(12), 1024-1033. SID. https://sid.ir/paper/36676/en

Vancouver: Copy

ABDOLLAHI MASOUD, HONARI HOSSIEN, NAZARIAN SHAHRAM, MASOUDI KERAHROUDI MEHDI. SUBCLONING AND EXPRESSION OF SO6 GENE, SAPONARIA OFFICINALIS PLANT IN E.COLI AND INVESTIGATION OF ANTIBODY TITER IN RATS. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;24(12):1024-1033. Available from: https://sid.ir/paper/36676/en

IEEE: Copy

MASOUD ABDOLLAHI, HOSSIEN HONARI, SHAHRAM NAZARIAN, and MEHDI MASOUDI KERAHROUDI, “SUBCLONING AND EXPRESSION OF SO6 GENE, SAPONARIA OFFICINALIS PLANT IN E.COLI AND INVESTIGATION OF ANTIBODY TITER IN RATS,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 24, no. 12, pp. 1024–1033, 2017, [Online]. Available: https://sid.ir/paper/36676/en

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