Effect of Vitamin E on Induction of Colonization of Spermatogonial Stem Cells in Vitro

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SALEHI F.; MOGHADDAM A.; RAHIMI FEYLI P.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Advanced Biomedical Sciences Year:2019 | Volume:9 | Issue:3 Page(s): 1613-1620

Download Full-Text

Persian Verion

View:

407

Download:

Cites:

Information Journal Paper

Title

Effect of Vitamin E on Induction of Colonization of Spermatogonial Stem Cells in Vitro

Author(s)

SALEHI F. | MOGHADDAM A. | RAHIMI FEYLI P. | Issue Writer Certificate

Keywords

Spermatogonial Stem cellQ1

Vitamin EQ2

In-VitroQ1

Abstract

Background & Objective: Spermatogonial Stem cells (SSCs) are undifferentiated germ cells that maintain spermatogenesis during lifetime by balancing between self-renewal and differentiation. The aim of this study was to investigate the effect of different concentrations of Vitamin E on Spermatogonial Stem cells colony formation. Materials & Methods: SSCs were isolated from testes of prepubertal lamb by two step enzymatic digestions then purified by differential plating. The cells were cultured for 10 days in 4 groups. Control group: Basic media (DMEM environment which contains 1% antibiotic and 5% FBS( and treatment groups were treated with 20, 40 and 80 μ mol/ml Vitamin E added to basic media respectively. Changing of culture media was performed every 72h. Colony number and diameter assay was done by inverted microscope. Identification of SSCs was performed by immunocytochemistry assay against PGP9. 5. Results: There were no significant difference in colony number and surface area between treatment groups and between treatment and control groups in days 4, 7and 10. Colony surface area significantly increased at day 10 compared to day 4 (P<0. 05). Conclusion: The results of this study showed that Vitamin E as an antioxidant does not have any significant effect on colony induction of SSCs in short term culture in vitro.

Cites

No record.

References

Cite

APA: Copy

SALEHI, F., MOGHADDAM, A., & RAHIMI FEYLI, P.. (2019). Effect of Vitamin E on Induction of Colonization of Spermatogonial Stem Cells in Vitro. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 9(3 ), 1613-1620. SID. https://sid.ir/paper/367527/en

Vancouver: Copy

SALEHI F., MOGHADDAM A., RAHIMI FEYLI P.. Effect of Vitamin E on Induction of Colonization of Spermatogonial Stem Cells in Vitro. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2019;9(3 ):1613-1620. Available from: https://sid.ir/paper/367527/en

IEEE: Copy

F. SALEHI, A. MOGHADDAM, and P. RAHIMI FEYLI, “Effect of Vitamin E on Induction of Colonization of Spermatogonial Stem Cells in Vitro,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 9, no. 3 , pp. 1613–1620, 2019, [Online]. Available: https://sid.ir/paper/367527/en

Related Journal Papers