EVALUATION OF ANTIMICROBIAL ACTIVITY OF THE ETHANOLIC EXTRACTS FROM Q.BRANTI AND Q.CASTANEIFOLIA FRUIT AGAINST SOME FOOD-BORNE PATHOGENS BY MICRODILUTION METHOD

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GHADERI GHAHFAROKHI M.; SADEGHI MAHOONAK A.; ALAMI M.; KHOMEIRI M.; MAMASHLOO S.

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Journal: Journal of Food Technology and Nutrition Year:2012 | Volume:9 | Issue:1 (33) Page(s): 81-94

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Title

EVALUATION OF ANTIMICROBIAL ACTIVITY OF THE ETHANOLIC EXTRACTS FROM Q.BRANTI AND Q.CASTANEIFOLIA FRUIT AGAINST SOME FOOD-BORNE PATHOGENS BY MICRODILUTION METHOD

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Keywords

ACORN FRUITQ3

ANTIMICROBIAL EFFECTQ2

ANTIRADICAL ACTIVITYQ3

PHENOLIC COMPOUNDQ2

Abstract

Introduction: Oak tree (Quercus) is a predominant plant genus in northern and central regions of Iran. ACORN FRUIT is a good source of PHENOLIC COMPOUNDs especially gallic acid and tannin. This study was designed to determine the PHENOLIC COMPOUNDs and evaluate the antiradical and antibacterial activities of ethanolic extracts from Q.branti var. persica and Q.castaneifolia var.castaneifoliafruit.Materials and Methods: After removal of internal husk and grounding the fruit, the ethanolic (70%) extracts were prepared according to re-extraction method. Polyphenol and flavonoid contents were measured spectrophotometrically. ANTIRADICAL ACTIVITY was evaluated against DPPH (2, 2-dipheny1-1-picrylhydrazyl) radicals. PHENOLIC COMPOUNDs were determind by the application of HPLC system equipped with a diode array detector. The antibacterial effects of the extracts were assessed on three gram positive and five gram negative bacteria by micro broth dilution technique using ELISA reader.Results: Total phenolic and flavonoid contents of ethanolic extract of castaneifolia variety were significantly higher than that of the extract of persica variety. The two extracts showed good antiradical activities that were comparable to BHA and BHT. Gallic, caffeic and cholorogenic acids were found in the two extracts while vanillin was detected only in extract ofcastaneifolia variety. The extracts showed good inhibitory and bactericidal effects against all investigated bacteria. The ranges of minimum bactericidal concentration (MBC) of the extracts were 0.615-5 and 1.25-5 mg/ml forcastaneifolia and persica respectively.Conclusion: This study suggested that ethanolic extract of ACORN FRUIT can be used potentially as a source of natural antioxidants and antimicrobial.

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APA: Copy

GHADERI GHAHFAROKHI, M., SADEGHI MAHOONAK, A., ALAMI, M., KHOMEIRI, M., & MAMASHLOO, S.. (2012). EVALUATION OF ANTIMICROBIAL ACTIVITY OF THE ETHANOLIC EXTRACTS FROM Q.BRANTI AND Q.CASTANEIFOLIA FRUIT AGAINST SOME FOOD-BORNE PATHOGENS BY MICRODILUTION METHOD. JOURNAL OF FOOD TECHNOLOGY AND NUTRITION, 9(1 (33)), 81-94. SID. https://sid.ir/paper/368302/en

Vancouver: Copy

GHADERI GHAHFAROKHI M., SADEGHI MAHOONAK A., ALAMI M., KHOMEIRI M., MAMASHLOO S.. EVALUATION OF ANTIMICROBIAL ACTIVITY OF THE ETHANOLIC EXTRACTS FROM Q.BRANTI AND Q.CASTANEIFOLIA FRUIT AGAINST SOME FOOD-BORNE PATHOGENS BY MICRODILUTION METHOD. JOURNAL OF FOOD TECHNOLOGY AND NUTRITION[Internet]. 2012;9(1 (33)):81-94. Available from: https://sid.ir/paper/368302/en

IEEE: Copy

M. GHADERI GHAHFAROKHI, A. SADEGHI MAHOONAK, M. ALAMI, M. KHOMEIRI, and S. MAMASHLOO, “EVALUATION OF ANTIMICROBIAL ACTIVITY OF THE ETHANOLIC EXTRACTS FROM Q.BRANTI AND Q.CASTANEIFOLIA FRUIT AGAINST SOME FOOD-BORNE PATHOGENS BY MICRODILUTION METHOD,” JOURNAL OF FOOD TECHNOLOGY AND NUTRITION, vol. 9, no. 1 (33), pp. 81–94, 2012, [Online]. Available: https://sid.ir/paper/368302/en

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