Effect of nano-hydroxyapatite on freezing ram spermatozoa

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BESHARATI F.; ROOSTAEI ALI MEHR M.

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Journal: Animal Production Research Year:2020 | Volume:8 | Issue:4 Page(s): 1-8

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Title

Effect of nano-hydroxyapatite on freezing ram spermatozoa

Author(s)

BESHARATI F. | ROOSTAEI ALI MEHR M. | Issue Writer Certificate

Keywords

SpermatozoaQ1

FreezingQ1

RamQ1

Nano-hydroxyapatite particlesQ1

Abstract

This experiment was designed to evaluate the protective effect of nano-hydroxyapatite against Freezing damages on Spermatozoa using five Rams. Ejaculates (N=30) were collected with three-day intervals between sessions for six times. Ejaculates were pooled and split into 12 parts at each session. The amount of 0. 01, 0. 02, 0. 05 or 0. 1% nano-hydroxyapatite and 3, 5 or 7% glycerol were added. Samples were frozen and stored for two weeks. After that two straws from each treatment for each replication (totally 144 straws) were thawed and incubated at 37 ° C for 6 h. Sperm motility, viability, membrane integrity and acrosome integrity were evaluated at 0, 3 and 6 h after thawing. Results showed that there was no interaction between nano-hydroxyapatite, glycerol and incubation time (P>0. 05). Sperm viability and acrosome integrity were not affected by different levels of nanohydroxyapatite particles (P>0. 05). There was no difference between the level of 0. 01 and 0. 1% nanohydroxyapatite particles on the total (21. 8 and 21. 5%, respectively) and progressive sperm motility (17. 8 and 17. 7 %, respectively; P>0. 05). Total and progressive sperm motility was the highest (24. 8 and 21. 0%, respectively) in the level of 0. 02% Nano-hydroxyapatite particles (P<0. 05). Membrane integrity was higher in the level of 0. 02% Nano-hydroxyapatite particles (52. 7%) than other levels (P<0. 05). Therefore, the addition of 0. 02% Nano-hydroxyapatite particles to the Ram semen extender improved the quality of frozen Spermatozoa.

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APA: Copy

BESHARATI, F., & ROOSTAEI ALI MEHR, M.. (2020). Effect of nano-hydroxyapatite on freezing ram spermatozoa. ANIMAL PRODUCTION RESEARCH, 8(4 ), 1-8. SID. https://sid.ir/paper/369074/en

Vancouver: Copy

BESHARATI F., ROOSTAEI ALI MEHR M.. Effect of nano-hydroxyapatite on freezing ram spermatozoa. ANIMAL PRODUCTION RESEARCH[Internet]. 2020;8(4 ):1-8. Available from: https://sid.ir/paper/369074/en

IEEE: Copy

F. BESHARATI, and M. ROOSTAEI ALI MEHR, “Effect of nano-hydroxyapatite on freezing ram spermatozoa,” ANIMAL PRODUCTION RESEARCH, vol. 8, no. 4 , pp. 1–8, 2020, [Online]. Available: https://sid.ir/paper/369074/en

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