Compare Antioxidant and cytotoxic effects of aqueous and hidroalcoholic extracts of Ruta graveolens on DU145 cell line

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Karavi A.; SHAHANIPOUR K.; MONAJEMI R.

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Journal: Journal of Animal Research Year:2017 | Volume:30 | Issue:3 Page(s): 0-0

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Title

Compare Antioxidant and cytotoxic effects of aqueous and hidroalcoholic extracts of Ruta graveolens on DU145 cell line

Author(s)

Karavi A. | SHAHANIPOUR K. | MONAJEMI R. | Issue Writer Certificate

Keywords

CytotoxicQ2

SodabQ1

DU-145 cell lineQ1

Antioxidant activityQ1

Total phenolQ1

Abstract

The prostate cancer is considered as the second most common malignancy in men after skin cancer. The alternative methods and novel compounds with lower side effects can be very important and critical for the patients due to high costs and many complications of radiotherapy, chemotherapy and invasive tissue surgery. The natural and herbal compounds have been noticed since they have variable advantages. The several herbal drugs have been identified up to now which have anticancer effect. Indeed, the plants are considered as an important source of antioxidant and phenolic compounds. The Sodab plant, Ruta graveolens, as a member of herbal drugs has a long consumption history in traditional medicine of Iran and different nations besides its significant therapeutic effects. The Sodab is a very valuable plant which has been named as “ a treatment for all diseases” and over 85 compounds of its different parts (leaves, stems, roots and seeds) has been recognized by gas-chromatography. The goals of this study were to investigate the Cytotoxic effect of aqueous and hydroalcoholic extracts of Sodab besides the comparison of these two extract effects on DU-145 cell line. In addition, the effect of this plant was studied on the prostate cancer cells. Furthermore, the antioxidant effect and the amount of phenolic compounds of Sodab extracts were evaluated. The Sodab plant was prepared from the Agriculture and Natural Resources Research Center of Isfahan. The next step was preparation of the water, hydroalcoholic and methanol extracts of this plant. The DU-145 cell line cultivation conditions were RPMI-1640 culture medium which contains 10% bovine serum, incubation with 5% CO2 and different concentrations of aqueous and hydroalcoholic extracts during 24, 48 and 72 hours of incubation. The MTT method was used in order to estimate the cells viability percent in the presence and absence of the extract and in the optical absorption was read with wavelength 540 nm by the ELISA device. The selected method for estimation of the Antioxidant activity was DPPH. In addition, the Total phenolic content of this plant extracts was analyzed by Folin Ciocalteu reagent. The gallic acid was used as the standard compound and the Total phenol was measured based on the milligram per gram of gallic acid. The Cytotoxic effect of Sodab aqueous and hydroalcoholic extracts was weak on DU-145 cell line while the Antioxidant activity and the phenolic content of this plant were significant. Key words: Cytotoxic, Sodab, DU-145 cell line, Antioxidant activity, Total phenol.

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APA: Copy

Karavi, A., SHAHANIPOUR, K., & MONAJEMI, R.. (2017). Compare Antioxidant and cytotoxic effects of aqueous and hidroalcoholic extracts of Ruta graveolens on DU145 cell line. JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 30(3 ), 0-0. SID. https://sid.ir/paper/369227/en

Vancouver: Copy

Karavi A., SHAHANIPOUR K., MONAJEMI R.. Compare Antioxidant and cytotoxic effects of aqueous and hidroalcoholic extracts of Ruta graveolens on DU145 cell line. JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2017;30(3 ):0-0. Available from: https://sid.ir/paper/369227/en

IEEE: Copy

A. Karavi, K. SHAHANIPOUR, and R. MONAJEMI, “Compare Antioxidant and cytotoxic effects of aqueous and hidroalcoholic extracts of Ruta graveolens on DU145 cell line,” JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 30, no. 3 , pp. 0–0, 2017, [Online]. Available: https://sid.ir/paper/369227/en

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