Callogenesis optimization and the effect of in vitro osmotic stress on Dianthus barbatus (L. ) calli

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Moshashaei abedin; Jowkar Mohammad mahdi; Frashadfar Ezatollah

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF PLANT PROCESS AND FUNCTION Year:2019 | Volume:7 | Issue:27 Page(s): 237-252

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

289

Download:

Cites:

Information Journal Paper

Title

Callogenesis optimization and the effect of in vitro osmotic stress on Dianthus barbatus (L. ) calli

Author(s)

Moshashaei abedin | Jowkar Mohammad mahdi | Frashadfar Ezatollah | Issue Writer Certificate

Keywords

BAPQ1

CatalaseQ2

Drought stressQ2

Glycine-BetaineQ2

NAAQ1

PeroxidaseQ1

Poly Ethylene GlycolQ1

Superoxide dismutaseQ2

Abstract

Dianthus barbatus is an important ornamental bedding plant in temperate regions with traditional medicinal applications. Water shortage and Drought stress are major limitations for landscape development and plant medicine production. Callogenesis is the key step for modern plant breeding techniques and in vitro Drought stress study defines the mechanisms of plant response to stress. Therefore this research focused on a) callogenesis optimization by different explants (leaf, stem and root) and plant growth regulators (BAP, NAA and 2, 4-D) and b) effect of osmotic stress (-1,-3,-6,-9 and-12 bar) induced by polyethylene glycol on D. barbatus calli. For callogenesis optimization, various criteria such as callogenesis percentage, calli colour, tissue type and growth besides chlorosis and necrosis were observed. In the second part, total protein and Glycine-Betaine content besides the activity of some free radical scavenger enzymes such as Superoxide dismutase, Catalase, Peroxidase were also measured during stress at various levels. Results indicate leaf as the most suitable explant and both 2µ M BAP + 4µ M NAA and 2µ M BAP + 6µ M NAA as the most suitable media supplements for callogenesis. As calli were subjected to osmotic stress, growth and weight of stressed calli decreased by osmotic stress increment. As osmotic pressure decreased (osmotic stress increased), total protein content decreased while Glycine-Betaine osmolyte increased. The activity of some free radical scavenger enzymes such as Superoxide dismutase, Catalase, Peroxidase also increased by osmotic stress increment.

Cites

No record.

References

No record.

Cite

APA: Copy

Moshashaei, abedin, Jowkar, Mohammad mahdi, & Frashadfar, Ezatollah. (2019). Callogenesis optimization and the effect of in vitro osmotic stress on Dianthus barbatus (L. ) calli. JOURNAL OF PLANT PROCESS AND FUNCTION, 7(27 ), 237-252. SID. https://sid.ir/paper/370787/en

Vancouver: Copy

Moshashaei abedin, Jowkar Mohammad mahdi, Frashadfar Ezatollah. Callogenesis optimization and the effect of in vitro osmotic stress on Dianthus barbatus (L. ) calli. JOURNAL OF PLANT PROCESS AND FUNCTION[Internet]. 2019;7(27 ):237-252. Available from: https://sid.ir/paper/370787/en

IEEE: Copy

abedin Moshashaei, Mohammad mahdi Jowkar, and Ezatollah Frashadfar, “Callogenesis optimization and the effect of in vitro osmotic stress on Dianthus barbatus (L. ) calli,” JOURNAL OF PLANT PROCESS AND FUNCTION, vol. 7, no. 27 , pp. 237–252, 2019, [Online]. Available: https://sid.ir/paper/370787/en

Related Journal Papers