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Information Journal Paper

Title

Cloning and expression of outer membrane protein 31 (Omp31) Brucella melitensis Rev1

Pages

  114-120

Abstract

 Brucellosis is a well-known infection among domestic animals caused by Brucella bacterium. Omp31 is outer membrane proteins that play important role in simulate CD4+ T and CD8+ T cells. In current study, Cloning, Expression and also phylogenic analysis of Omp31 gene as a candidate for designing subunit vaccine against Brucella was investigated. Amplifying was performed using specific primers. Cloning of this gene was performed using pMB57R/T vector in TOP10F`strain of E. coli. Also, pET32a vector used for Expression. Omp31 gene with 723 bp was amplified successfully. It, then, was coloned using TA Cloning approach within Cloning vector. Expression of this gene has done in pET32a vector by inducing IPTG. Results confirmed with sequencing and SDS-PAGE. According to this result, we can propose this gene as a candidate for designing subunit vaccine against Brucella in future study.

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    APA: Copy

    YOUSEFI, S., TAHMOORESPUR, M., & SEKHAVATI, M.H.. (2018). Cloning and expression of outer membrane protein 31 (Omp31) Brucella melitensis Rev1. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, 14(2 ), 114-120. SID. https://sid.ir/paper/372444/en

    Vancouver: Copy

    YOUSEFI S., TAHMOORESPUR M., SEKHAVATI M.H.. Cloning and expression of outer membrane protein 31 (Omp31) Brucella melitensis Rev1. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL[Internet]. 2018;14(2 ):114-120. Available from: https://sid.ir/paper/372444/en

    IEEE: Copy

    S. YOUSEFI, M. TAHMOORESPUR, and M.H. SEKHAVATI, “Cloning and expression of outer membrane protein 31 (Omp31) Brucella melitensis Rev1,” SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL, vol. 14, no. 2 , pp. 114–120, 2018, [Online]. Available: https://sid.ir/paper/372444/en

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