DEVELOPMENT OF TRANSGENIC MICE HARBORING OVINE BETA LACTOGLOBULINCALCITONIN TRANSGENE

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NIAVARANI A.R.; DEHGHANIZADE S.; KARIMI M.; ZEYNALI S.

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Journal: Koomesh Year:2005 | Volume:6 | Issue:3 Page(s): 207-215

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Information Journal Paper

Title

DEVELOPMENT OF TRANSGENIC MICE HARBORING OVINE BETA LACTOGLOBULINCALCITONIN TRANSGENE

Author(s)

NIAVARANI A.R. | DEHGHANIZADE S. | KARIMI M. | ZEYNALI S. | Issue Writer Certificate

Keywords

TRANSGENIC MICEQ4

MILKQ2

CALCITONINQ4

BETA-LACTOGLOBULINQ4

RECOMBINANT PROTEINQ4

FUSION PROTEINQ4

Abstract

Introduction: Expression of foreign proteins in mammalian MILK is becoming a widespread strategy for high-level production of recombinant pharmaceuticals, especially those with the most complex post-translational modifications. We have attempted in this project to develop TRANSGENIC MICE harboring a transgene driving mammary gland expression of hybrid human salmon calcionin. Materials and Methods: A MILK-specific ovine BETA-LACTOGLOBULIN (oBLG) promoter was used to drive expression of recombinant CALCITONIN in mouse MILK. A gene construct was generated, consisting of 10.7kbp of the oBLG gene including its promoter and 3' flanking region with the CALCITONIN coding sequences inserted in-frame into the oBLG fifth exon. The gene construct was purified using CsCl gradient, released from vector, and gel-purified. After appropriate dilution, it was microinjected into recently-fertilized mouse oocytes. These oocytes then were transferred to pseudo-pregnant foster mice. Results: Forty one pups were born from foster mice, which were genotyped using PCR, slot blotting, and Southern blotting. Among 9 mice which showed positive PCR results, 6 mice resulted in pups with positive PCR tests. All six families transmitted the transgene to first and second generation. Conclusion: As the main criteria for considering a mouse as transgenic is transgene transmission to the next generation, all 6 mice which stably transmitted their transgene to progeny are considered as transgenic founders and constitute independent transgenic lines.

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APA: Copy

NIAVARANI, A.R., DEHGHANIZADE, S., KARIMI, M., & ZEYNALI, S.. (2005). DEVELOPMENT OF TRANSGENIC MICE HARBORING OVINE BETA LACTOGLOBULINCALCITONIN TRANSGENE. KOOMESH, 6(3), 207-215. SID. https://sid.ir/paper/37414/en

Vancouver: Copy

NIAVARANI A.R., DEHGHANIZADE S., KARIMI M., ZEYNALI S.. DEVELOPMENT OF TRANSGENIC MICE HARBORING OVINE BETA LACTOGLOBULINCALCITONIN TRANSGENE. KOOMESH[Internet]. 2005;6(3):207-215. Available from: https://sid.ir/paper/37414/en

IEEE: Copy

A.R. NIAVARANI, S. DEHGHANIZADE, M. KARIMI, and S. ZEYNALI, “DEVELOPMENT OF TRANSGENIC MICE HARBORING OVINE BETA LACTOGLOBULINCALCITONIN TRANSGENE,” KOOMESH, vol. 6, no. 3, pp. 207–215, 2005, [Online]. Available: https://sid.ir/paper/37414/en

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