Study the dependence of Ciprofloxacin-induced mutagenesis on UmuC in Escherichia coli

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Nourbakhsh Rezaei S.M.; POURAHMAD R.; MAHZOONIEH M.R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Molecular and Cellular Researches Year:2019 | Volume:32 | Issue:4 Page(s): 566-577

Download Full-Text

Persian Verion

View:

323

Download:

Cites:

Information Journal Paper

Title

Study the dependence of Ciprofloxacin-induced mutagenesis on UmuC in Escherichia coli

Author(s)

Nourbakhsh Rezaei S.M. | POURAHMAD R. | MAHZOONIEH M.R. | Issue Writer Certificate

Keywords

CiprofloxacinQ1

mutagenesisQ2

UmuCQ1

Escherichia coli bacteriaQ1

Abstract

Ciprofloxacin is a fluoroquinolone effective against Gram-negative bacteria such as Escherichia coli. In Escherichia coli exposure to Ciprofloxacin resulted in mutagenesis and increase in antibiotic resistance. In addition, exposure to UV radiation induces the transcription of UmuC. The importance of other proteins in mutagenesis has not been investigated. Thus, study mutagenesis in the absence of UmuC is necessary. The aim of this study was to study the dependence of ciproﬂ oxacin-induced mutagenesis on UmuC in Escherichia coli. In this research, Escherichia coli UmuC- strain and wild type strain + (UmuC ) was used and resistance to Ciprofloxacin was induced stepwise. To determine the MIC of Ciprofloxacin serial dilution method in broth medium was used. The mutation frequency was determined in the presence of Ciprofloxacin and UV rays. The results showed that in the absence of UmuC mutants with low and medium resistance can be produced from UmuC- parent strain but high resistant mutants were not obtained and the mutation frequency was low in the presence of Ciprofloxacin in UmuC- mutant, but higher than that of parent and wild type strains. Mutation frequency in the presence of UV rays in UmuC- mutant was lower than that in wild type strain. In conclusion, UmuC is required to generate mutants with high resistance to Ciprofloxacin. The frequency of mutation suggests that other factors might be effective.

Cites

No record.

References

No record.

Cite

APA: Copy

Nourbakhsh Rezaei, S.M., POURAHMAD, R., & MAHZOONIEH, M.R.. (2019). Study the dependence of Ciprofloxacin-induced mutagenesis on UmuC in Escherichia coli. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 32(4 ), 566-577. SID. https://sid.ir/paper/374375/en

Vancouver: Copy

Nourbakhsh Rezaei S.M., POURAHMAD R., MAHZOONIEH M.R.. Study the dependence of Ciprofloxacin-induced mutagenesis on UmuC in Escherichia coli. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2019;32(4 ):566-577. Available from: https://sid.ir/paper/374375/en

IEEE: Copy

S.M. Nourbakhsh Rezaei, R. POURAHMAD, and M.R. MAHZOONIEH, “Study the dependence of Ciprofloxacin-induced mutagenesis on UmuC in Escherichia coli,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 32, no. 4 , pp. 566–577, 2019, [Online]. Available: https://sid.ir/paper/374375/en

Related Journal Papers