IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS BEIJING GENOTYPE USING THREE DIFFERENT MOLECULAR METHODS

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TAJ ALDIN E.; FARNIA P.; KARGAR MOHAMMAD; NOUROUZI JAMILEH; AHMADI MOJTABA; KAZEMPOUR DIZAJI M.; MASJEDI M.R.; VELAYATI A.A.

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Journal: Koomesh Year:2009 | Volume:11 | Issue:1 (33) Page(s): 7-14

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Title

IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS BEIJING GENOTYPE USING THREE DIFFERENT MOLECULAR METHODS

Author(s)

Keywords

SPOLIGOTYPINGQ3

VNTR TYPINGQ3

RFLPQ3

MYCOBACTERIUM TUBERCULOSISQ3

BEIJING GENOTYPEQ3

Abstract

Introduction: Beijing strains constitute more than 1/4 of MYCOBACTERIUM TUBERCULOSIS (MT) genotypes. BEIJING GENOTYPE is considered an important genotype because of its reasonable characteristics such as association with multi-drugs resistance TB. The aim of this study was to identify the genetic pattern of MT BEIJING GENOTYPE using SPOLIGOTYPING, variable number tandem repeat (VNTR) typing and RFLP-IS6110 methods.Materials and Methods: 238 MT culture positive specimens (2007-2008) were genotyped by SPOLIGOTYPING. Thereafter, the isolated BEIJING GENOTYPE was subjected to VNTR TYPING and RFLP analysis. The results of SPOLIGOTYPING were analyzed using SPOLDB4 database. VNTR TYPING was used to identify alleles diversity in 9 locus (MPTR-A, ETR-A, ETR-B, ETR-C, ETR-D, ETR-E, ETR-F, QUB11B, QUB3232) of the isolated Beijing strains. The allelic diversity of VNTR was measured using Hunter Gaston Index (HGI).Results: The SPOLIGOTYPING of MT isolates revealed the following 8 patterns: Haarlem (27.7%), CAS1 (25.2%), EAI3 (21.8%), CAS2 (6.7%), T1 (6.3%), Beijing (5.5%), U (5%), T (0.4), EAI2 (1.2%). By VNTR TYPING, the QUB3232 locus was identified as the most distinctive (HGI≥0.6); ETR-F, ETR-E and QUB11b loci; as median distinctive loci (HGI≥0.4-0.6) and other loci as the weakest distinctive locus for Beijing families. The Beijing strains demonstrated diverse patterns in RFLP, 13.13 (100%) and VNTR 10.13 (77%).Conclusion: The ariety of genetic patterns revealed the reactivation in the studied population. As RFLP method is a time consuming and costly effective method, VNTR and SPOLIGOTYPING method can be used as alternative methods for molecular epidemiology of MT.

Cites

MOLECULAR TYPING OF MYCOBACTERIUM TUBERCULOSIS STRAINS ISOLATED FROM PATIENTS IN MARKAZI PROVINCE

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APA: Copy

TAJ ALDIN, E., FARNIA, P., KARGAR, MOHAMMAD, NOUROUZI, JAMILEH, AHMADI, MOJTABA, KAZEMPOUR DIZAJI, M., MASJEDI, M.R., & VELAYATI, A.A.. (2009). IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS BEIJING GENOTYPE USING THREE DIFFERENT MOLECULAR METHODS. KOOMESH, 11(1 (33)), 7-14. SID. https://sid.ir/paper/37494/en

Vancouver: Copy

TAJ ALDIN E., FARNIA P., KARGAR MOHAMMAD, NOUROUZI JAMILEH, AHMADI MOJTABA, KAZEMPOUR DIZAJI M., MASJEDI M.R., VELAYATI A.A.. IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS BEIJING GENOTYPE USING THREE DIFFERENT MOLECULAR METHODS. KOOMESH[Internet]. 2009;11(1 (33)):7-14. Available from: https://sid.ir/paper/37494/en

IEEE: Copy

E. TAJ ALDIN, P. FARNIA, MOHAMMAD KARGAR, JAMILEH NOUROUZI, MOJTABA AHMADI, M. KAZEMPOUR DIZAJI, M.R. MASJEDI, and A.A. VELAYATI, “IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS BEIJING GENOTYPE USING THREE DIFFERENT MOLECULAR METHODS,” KOOMESH, vol. 11, no. 1 (33), pp. 7–14, 2009, [Online]. Available: https://sid.ir/paper/37494/en

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