DETECTION AND CLONING OF DESULFURIZATION OPERON SOX(DSZA&B) IN RHODOCOCCUS FMF NATIVE BACTERIUM

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AKBARZADEH S.; RAHEB J.

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Journal: Koomesh Year:2004 | Volume:5 | Issue:3-4 Page(s): 171-177

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Title

DETECTION AND CLONING OF DESULFURIZATION OPERON SOX(DSZA&B) IN RHODOCOCCUS FMF NATIVE BACTERIUM

Author(s)

AKBARZADEH S. | RAHEB J. | Issue Writer Certificate

Keywords

RHODOCOCCUS SP.Q4

RHODOCOCCUS ERYTHROPOLIS IGTS8Q3

BIODESULFURIZATIONQ3

DIBENZOTHIOPHENEQ4

Abstract

Introduction: Native Rhodococcus FMF bacterium was selected among several isolated Iranian bacterium. Primary studies have shown that the bacterium use DIBENZOTHIOPHENE as a sulfur source. Since desulfurization is taken by a biochemical pathway, the present project was designed to detect, clone and sequence of desulfurization genes of the bacterium. Materials and Methods: Desulfurization operon was isolated from RHODOCOCCUS ERYTHROPOLIS IGTS8 and was used as a probe for detection of desulfurization 4S pathway genes by southern blotting technique. Specific primers were designed and the dszA and B genes were amplified by polymerase chain reaction (PCR). PCR products were recovered from agarose gel and were cloned into the PTZ57R vector. Results: In this study, recombinant PTZAB57R vector was constructed by the insertion of dszA and B genes into the PTZ57R vector. The clones were confirmed by restriction digestion analysis. The PTZAB57R was extracted by large-scale method and was sequenced (MWG DNA Biotech).Conclusion: Comparison of sequences of dszA and B genes from native Rhodococcus FMF bacterium with RHODOCOCCUS SP. IGTS8 bacterium were shown complete identity between them, which show that desulfurization pathway is conserved in this bacterium.

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APA: Copy

AKBARZADEH, S., & RAHEB, J.. (2004). DETECTION AND CLONING OF DESULFURIZATION OPERON SOX(DSZA&B) IN RHODOCOCCUS FMF NATIVE BACTERIUM. KOOMESH, 5(3-4), 171-177. SID. https://sid.ir/paper/37568/en

Vancouver: Copy

AKBARZADEH S., RAHEB J.. DETECTION AND CLONING OF DESULFURIZATION OPERON SOX(DSZA&B) IN RHODOCOCCUS FMF NATIVE BACTERIUM. KOOMESH[Internet]. 2004;5(3-4):171-177. Available from: https://sid.ir/paper/37568/en

IEEE: Copy

S. AKBARZADEH, and J. RAHEB, “DETECTION AND CLONING OF DESULFURIZATION OPERON SOX(DSZA&B) IN RHODOCOCCUS FMF NATIVE BACTERIUM,” KOOMESH, vol. 5, no. 3-4, pp. 171–177, 2004, [Online]. Available: https://sid.ir/paper/37568/en

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